The major reasons for more usage of molecular fluorescence spectrometry in comparison to molecular phosphorescence spectrometry should be discussed. Concept introduction: Phosphorescence spectrometry is closely related to fluorescence. The transition from excited to ground state takes time in this type of photoluminescence process.

Question

The levels of Vitamin B1 in a sample of milk was determined using the Standard Addition method and technique of fluorescence spectroscopy. 18.0 ml of the milk sample was diluted to 20.0 ml using distilled water and then the fluorescence was measured. The resulting signal was 210 units. A spike was made by taking 18.0 ml of the same milk sample, adding 1.0 ml of a 6.0 ppm Vitamin B1 standard solution, and then diluting to 20.0 ml using distilled water. The signal of the spiked milk was 540 units. Calculate the concentration of Vitamin B1 in the original milk sample (in ppm). Report to 3 decimal places.

Answer 1

Question

Chapter 15, Problem 15.6QAP

Interpretation Introduction

Interpretation:

The major reasons for more usage of molecular fluorescence spectrometry in comparison to molecular phosphorescence spectrometry should be discussed.

Concept introduction:

Phosphorescence spectrometry is closely related to fluorescence. The transition from excited to ground state takes time in this type of photoluminescence process.

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The levels of Vitamin B1 in a sample of milk was determined using the Standard Addition method and technique of fluorescence spectroscopy. 18.0 ml of the milk sample was diluted to 20.0 ml using distilled water and then the fluorescence was measured. The resulting signal was 210 units. A spike was made by taking 18.0 ml of the same milk sample, adding 1.0 ml of a 6.0 ppm Vitamin B1 standard solution, and then diluting to 20.0 ml using distilled water. The signal of the spiked milk was 540 units. Calculate the concentration of Vitamin B1 in the original milk sample (in ppm). Report to 3 decimal places.

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