Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 14.5, Problem 3CC
Retrieve, Infer, Apply
Describe the events that occur with E. coli in each of the following growth conditions: in a medium containing glucose but not lactose; in a medium containing both sugars; in a medium containing lactose but no glucose; and in a medium containing neither sugar.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
What is the expected yield for plasma membranes isolated by sucrose gradient centrifugation from mouse tissues?
Discuss the suitability of using the PFR as a bioreactor for algae growth.
in isolating ribosomes from a yield sample, describe the ideal type of centrifugation for this separation technique based on the following:*Main type of centrifuge *Subtype centrifuge * Speed range *Operational temperature range*Type of Centrifugation
Chapter 14 Solutions
Prescott's Microbiology
Ch. 14.2 - MICRO INQUIRY In what way is on inducer molecule...Ch. 14.2 - MICRO INQUIRY Is allolactose a corepressor or...Ch. 14.2 - Retrieve, Infer, Apply Many genes and operons are...Ch. 14.2 - Prob. 2CCCh. 14.2 - Prob. 3CCCh. 14.3 - MICRO INQUIRY How does this attenuation respond to...Ch. 14.4 - MICRO INQUIRY How does inhibition of translation...Ch. 14.4 - Prob. 1CCCh. 14.4 - Prob. 2CCCh. 14.4 - Prob. 3CC
Ch. 14.4 - Retrieve, Infer, Apply How are translational...Ch. 14.4 - Prob. 5CCCh. 14.5 - MICRO INQUIRY Relative to each promoter, where...Ch. 14.5 - MICRO INQUIRY For what other compounds would you...Ch. 14.5 - Prob. 1CCCh. 14.5 - Retrieve, Infer, Apply What is diauxic growth?...Ch. 14.5 - Retrieve, Infer, Apply Describe the events that...Ch. 14.5 - Retrieve, Infer, Apply E. coli has two phosphate...Ch. 14.5 - Prob. 5CCCh. 14.6 - Prob. 1MICh. 14.6 - MICRO INQUIRY Why does V. harveyi make three...Ch. 14.6 - Prob. 3MICh. 14.6 - Prob. 1CCCh. 14.6 - Prob. 2CCCh. 14.6 - Prob. 3CCCh. 14.6 - Retrieve, Infer, Apply Why might bacteria use...Ch. 14.6 - Prob. 5CCCh. 14.6 - Prob. 6CCCh. 14 - Prob. 1RCCh. 14 - Prob. 2RCCh. 14 - Prob. 3RCCh. 14 - Prob. 4RCCh. 14 - Attenuation affects anabolic pathways, whereas...Ch. 14 - Describe the phenotype of the following E. coli...Ch. 14 - Prob. 3ALCh. 14 - What would be the phenotype of a B. subtilis...Ch. 14 - Propose a mechanism by which a cell might sense...Ch. 14 - Prob. 6ALCh. 14 - Prob. 7ALCh. 14 - Prob. 8ALCh. 14 - Prob. 9AL
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Bacterial Identification Virtual Lab Add the Master Mix and answer the following questions: 13. What does the Master Mix contain? 14. What are primers? Why is a primer added? 15. Once the primers bind, what occurs next? 16. What does "highly conserved" mean? 17. Why are highly conserved regions important in this lab?arrow_forwardDo you think you can also use Invitrogen PureLink Genomic DNA Mini Kit to obtain chromosomal DNA from a pure culture of archaea that is hyperthermophilic, alkaliphilic and halophilic in nature? Why or why not? What features of the kit make it effective or not effective for this scenario?arrow_forwardHypothetically make the Dichotomous key for Micrococcus luteus, Bacillus subtilis, Bacillus, Megaterium, Bacillus cereus, E.coli, Serratia Marcescens, and Enterobacter aerogenes based on colony morphology. results of catalase test, (lactose, sucrose and glucose fermentation tests) and other biochemical tests for its accurate identification.arrow_forward
- Theoretical Data Following the modified protocol for the isolation of Escherichia coli bacteriophage of Encabo (2018) presented below, compute for the pfu/ml of the chloroform-treated lysate. 1ml 1ml 1ml 1ml 1 Chloroform-treated lysate 1ml Dilution 10-3 10-4 10-5 0 0 0 0 0 9ml 9ml 9ml 10-1 10-² 10-3 Empty sterile tubes Incubate inside ref for 15-20min + 0.1 ml lysate-E.coli mix 0.1 ml 9ml 9ml 104 10.5 // 0.1 ml 0.1 ml +0.5 ml E. coli ↓↓↓↓↓↓ Incubate O/N at 35°C, observe for plaques Molten soft agar overlay B. Quantification of the Infective E. coli other Bacteriophage Particles in the Raw Sewage Sample Volume of Stock Plated (in ml) Bottom agar No. of Plaque-Forming Units (pfu) Ave. A B 254 265 132 110 11 23arrow_forwardDifferentiate the Guanidium-thiocyanate phenol-chloroform method from the silica membrane based technology. What are the advantages or disadvantages of using commercially available kits compared to conventional RNA extraction techniques?arrow_forwardDRAW IT Draw the following growth curves for E. coli, starting with 100 cells with a generation time of 30 minutes at 35°C 60 minutes at 20°C, and 3 hours at 5°C. (A) The cells are incubated for 5 hours at 35°C. (B) After 5 hours, the temperature is changed to 20°C for 2 hours. (C) After 5 hours at 35°C, the temperature is changed to 5°C for 2 hours followed by 35°C for 5 hours. *arrow_forward
- Q1: Why are E.coli cells subjected to heat shock induction when the optical density of the bacterial culture has reached 0.6-0.8? Q2: Why are these wash steps critical for the preparation of protein samples used in 2D gel electrophoresis?arrow_forwardpresent briefly the principle of size exclusion chromatography. give a brief description of the use of protein assays.arrow_forwardin isolating ribosomes from a yield sample, describe the ideal type of centrifugation for this separation technique based on the following:*Type of Centrifugation* Type of fraction *Give 2 advantages of using this type of centrifuge.*Give 2 disadvantages of using this type of centrifuge.arrow_forward
- In growing E.coli, why is that (reasons) they do not grow after doubling time under 20 degrees celsius and under 37 degrees celsius, the number of colonies after doubling time has decreased? provide reliable sources (links).arrow_forwardCould any one provide me with the protocol for Plasma Membrane Isolation via sucrose density gradient ultracentrifuagation?arrow_forwardAeroderma pigmentosum patients have a 10,000-fold increase in the risk of developing Skin cancer. These patients have to avoid exposure to UV light. Please explain why. To answer the question please: I) name the process that is affected and provide a scheme; 2) indicate template, substrates, sources of energy that are involved in the process; 3) propose the consequences of the process.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
cell culture and growth media for Microbiology; Author: Scientist Cindy;https://www.youtube.com/watch?v=EjnQ3peWRek;License: Standard YouTube License, CC-BY