Campbell Biology in Focus (2nd Edition)
2nd Edition
ISBN: 9780321962751
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece
Publisher: PEARSON
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Question
Chapter 13.4, Problem 1CC
Summary Introduction
To identify:
The sequence and the types of the bonds that are being cleaved in the sequence of the DNA by the restriction enzyme PvuI namely:
Concept introduction:
The enzyme PvuI cuts in between the T-C
The types of the bonds that will be cleaved in between the nucleotides are the phosphate bonds between the pentose sugar and the nucleotide connecting the next nucleotide in the sequence.
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MAKE CONNECTIONS The restriction site for an enzymecalled PvuI is the following sequence:5’-CGATCG-3’3’-GCTAGC-5’
3
The restriction endonuclease Pstl cuts DNA symmetrically on both strands at the
СТGCAG
sequence:
GACGTC
On the resulting DNA fragments are left 3' overhanging ends of 4 nucleotides. PstI cleaves
the phosphodiester backbone with a-type specificity. In short-hand notation, showing the
identity of phosphates on the ends, draw the two fragments that result from PstI digestion of
the following double-stranded oligonucleotide:
5'-рAATTGCTAСTGCAGAACCGG-3'
3'-ТТAАCGATGACGTCTTGGСС--5'
Sensors detect the flash of
light.
DNA polymerase
Unused
deoxyribonucleotides are
cleaved by apyrase.
ATP is consumed by
luciferase and light is
emitted.
AMP and PP, are
converted into ATP by
sulfurylase.
Template strand
Growing strand
3' TAGGCCTACACTTACGCGAATGT 5'
5' ATCCGGAT 3'
dGTP
dNTPs
dNDPs
dNMPs
+ P₁
PP₁
ATP
[1]
Chapter 13 Solutions
Campbell Biology in Focus (2nd Edition)
Ch. 13.1 - Given a polynucleotide sequence such as GAATTC,...Ch. 13.1 - Prob. 2CCCh. 13.2 - What role does base pairing play in the...Ch. 13.2 - Make a table listing the functions of seven...Ch. 13.2 - MAKE CONNECTIONS What is the relationship between...Ch. 13.3 - Describe the structure of a nucleosome, the basic...Ch. 13.3 - What two properties, one structural and one...Ch. 13.4 - Prob. 1CCCh. 13.4 - DRAW IT One strand of a DNA molecule has the...Ch. 13.4 - Describe the role of complementary base pairing...
Ch. 13 - In his work with pneumonia-causing bacteria and...Ch. 13 - What is the basis for the difference in how the...Ch. 13 - In analyzing the number of different bases in a...Ch. 13 - The elongation of the leading strand during DNA...Ch. 13 - Prob. 5TYUCh. 13 - Prob. 6TYUCh. 13 - Prob. 7TYUCh. 13 - Prob. 8TYUCh. 13 - Prob. 9TYUCh. 13 - MAKE CONNECTIONS Although the proteins that cause...Ch. 13 - Prob. 12TYUCh. 13 - FOCUS ON EVOLUTION Some bacteria may be able to...Ch. 13 - FOCUS ON ORGANIZATION The continuity of life is...
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