Foundations in Microbiology
10th Edition
ISBN: 9781259705212
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Chapter 13.1, Problem 2ELO
Summary Introduction
Introduction:
There are few terms associated with the infectious diseases that are commonly used.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 13 Solutions
Foundations in Microbiology
Ch. 13.1 - Describe some of the major interactions between...Ch. 13.1 - Prob. 2ELOCh. 13.1 - Discuss the characteristics of the normal...Ch. 13.1 - Briefly relate the sources and conditions that...Ch. 13.1 - Identify which bodily sites remain free of living...Ch. 13.1 - Prob. 6ELOCh. 13.1 - Prob. 1CYPCh. 13.1 - Prob. 2CYPCh. 13.1 - Prob. 3CYPCh. 13.1 - Prob. 4CYP
Ch. 13.1 - Prob. 5CYPCh. 13.1 - Differentiate between transient and resident...Ch. 13.1 - Explain the factors that cause variations in the...Ch. 13.2 - Review the main stages in the development of an...Ch. 13.2 - Prob. 8ELOCh. 13.2 - Prob. 9ELOCh. 13.2 - Prob. 10ELOCh. 13.2 - Prob. 11ELOCh. 13.2 - Identify and discuss invasive factors and...Ch. 13.2 - Prob. 13ELOCh. 13.2 - Explain several ways in which true pathogens...Ch. 13.2 - Distinguish between pathogenicity and virulence.Ch. 13.2 - Prob. 10CYPCh. 13.2 - Prob. 11CYPCh. 13.2 - Prob. 12CYPCh. 13.2 - Describe several components of pathogens that are...Ch. 13.2 - Prob. 14CYPCh. 13.2 - Prob. 15CYPCh. 13.2 - Define toxigenicity and summarize the main...Ch. 13.2 - Prob. 17CYPCh. 13.3 - Describe the clinical stages of infection.Ch. 13.3 - Use key terms to describe different patterns of...Ch. 13.3 - Prob. 16ELOCh. 13.3 - Prob. 17ELOCh. 13.3 - Explain what is happening during each stage of...Ch. 13.3 - Prob. 19CYPCh. 13.3 - Name some examples of infections and their portals...Ch. 13.3 - 21. Using terminology from this section's “Guide...Ch. 13.4 - Define epidemiology, and summarize the major goals...Ch. 13.4 - Prob. 19ELOCh. 13.4 - Prob. 20ELOCh. 13.4 - Prob. 21ELOCh. 13.4 - Prob. 22ELOCh. 13.4 - Prob. 23ELOCh. 13.4 - Prob. 22CYPCh. 13.4 - Prob. 23CYPCh. 13.4 - Prob. 24CYPCh. 13.4 - Prob. 25CYPCh. 13.4 - Prob. 26CYPCh. 13.4 - What is epidemiologically and medically important...Ch. 13.5 - Prob. 24ELOCh. 13.5 - Prob. 25ELOCh. 13.5 - Summarize the steps in Koch’s postulates, and...Ch. 13.5 - Prob. 27ELOCh. 13.5 - Prob. 28ELOCh. 13.5 - Prob. 28CYPCh. 13.5 - Prob. 29CYPCh. 13.5 - Prob. 30CYPCh. 13.5 - Prob. 31CYPCh. 13.5 - Prob. 32CYPCh. 13.5 - Outline the major factors involved in...Ch. 13.L1 - Prob. 1MCQCh. 13.L1 - Prob. 2MCQCh. 13.L1 - Prob. 3MCQCh. 13.L1 - Prob. 4MCQCh. 13.L1 - Prob. 5MCQCh. 13.L1 - Prob. 6MCQCh. 13.L1 - Prob. 7MCQCh. 13.L1 - The presence of a few bacteria in the blood is...Ch. 13.L1 - Prob. 9MCQCh. 13.L1 - A/an ______ is a passive animal transporter of...Ch. 13.L1 - Prob. 11MCQCh. 13.L1 - Prob. 12MCQCh. 13.L1 - Prob. 13MCQCh. 13.L1 - A positive antibody test for HIV would be a...Ch. 13.L1 - Prob. 15MCQCh. 13.L1 - Prob. 16MCQCh. 13.L1 - Prob. 1CSRCh. 13.L1 - Prob. 2CSRCh. 13.L1 - Prob. 3CSRCh. 13.L1 - Prob. 1WCCh. 13.L1 - Prob. 2WCCh. 13.L1 - Prob. 3WCCh. 13.L1 - Prob. 4WCCh. 13.L1 - Prob. 5WCCh. 13.L1 - Prob. 6WCCh. 13.L1 - Prob. 7WCCh. 13.L1 - a. Outline the five types of clinical isolation....Ch. 13.L1 - Complete the following table. Chemical makeup...Ch. 13.L2 - Discuss the relationship between the vaginal...Ch. 13.L2 - Prob. 2CTCh. 13.L2 - How could the microbiome cause some infections to...Ch. 13.L2 - Each of the nine patient specimens listed below...Ch. 13.L2 - Prob. 5CTCh. 13.L2 - Prob. 6CTCh. 13.L2 - Prob. 7CTCh. 13.L2 - a. Suggest several reasons why respiratory,...Ch. 13.L2 - Summarize the epidemiological findings in the...Ch. 13.L2 - Looking at figure 13.20b. Which pattern of...Ch. 13.L2 - Prob. 1VCCh. 13.L2 - Observe the following maps (a)-(c) of three...Ch. 13.L2 - Prob. 3VC
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