Becker's World of the Cell (9th Edition)
Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 13, Problem 13.8PS

Stressed Out. It is now possible, via nanoengineering, to attach magnetic beads to the surface of large cells. Using the beads, you apply force to measure how mechanically stiff the cells are. What effect would you predict acrylamide treatment would have on the mechanical rigidity of a keratinocyte (skin cell)? Explain your answer.

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Movement of Dyes in an Agarose Gel.This problem tests your ability to understand basic concepts of agarose gel electrophoresis.The gel is loaded from lane 1 at the top to lane 7 at the bottom with: bromophenol blue, safranin O, orange G, crystal violet, xylene cyanol, methylene blue, and the unknown.Lines have been added to the gel to help you locate the center of the wells.   Which known dye samples are positively charged?  Which known dyes are in the unknown sample in lane 7?
Troubleshooting gels. What effect will be observed on the stained bands of protein in apolyacrylamide gel if…a. …the samples are not incubated at 95˚C prior to loading?b. …the gel is run at too high or too low a voltage?c. …too much protein is loaded?
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