Essentials of Biology (5th International Edition)
5th Edition
ISBN: 9781259660269
Author: Sylvia S. Mader, Dr., Michael Windelspecht
Publisher: Mcgraw-Hill
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Textbook Question
Chapter 12.1, Problem 1CYP
Explain the role of the bacterial plasmid in recombinant DNA technology.
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Chapter 12 Solutions
Essentials of Biology (5th International Edition)
Ch. 12.1 - Prob. 1LOCh. 12.1 - Explain the purpose of the polymerase chain...Ch. 12.1 - Prob. 3LOCh. 12.1 - Prob. 4LOCh. 12.1 - Explain the role of the bacterial plasmid in...Ch. 12.1 - Prob. 2CYPCh. 12.1 - Prob. 3CYPCh. 12.1 - Prob. 4CYPCh. 12.1 - Prob. 1ACh. 12.1 - Prob. 2A
Ch. 12.1 - Prob. 3ACh. 12.2 - Prob. 1LOCh. 12.2 - Prob. 2LOCh. 12.2 - Prob. 1CYPCh. 12.2 - Summarize the origins of both embryonic and adult...Ch. 12.2 - Prob. 3CYPCh. 12.2 - Prob. 4ACh. 12.2 - Prob. 5ACh. 12.2 - Prob. 6ACh. 12.3 - Prob. 1LOCh. 12.3 - Prob. 2LOCh. 12.3 - Prob. 1CYPCh. 12.3 - Prob. 2CYPCh. 12.3 - Prob. 3CYPCh. 12.3 - Prob. 7ACh. 12.3 - Prob. 8ACh. 12.4 - Prob. 1LOCh. 12.4 - Prob. 2LOCh. 12.4 - Prob. 3LOCh. 12.4 - Prob. 1CYPCh. 12.4 - Prob. 2CYPCh. 12.4 - Prob. 3CYPCh. 12.4 - Prob. 9ACh. 12.4 - Prob. 10ACh. 12 - Prob. 1BYBCh. 12 - Prob. 2BYBCh. 12 - Prob. 3BYBCh. 12 - Prob. S11.1ABYBCh. 12 - Prob. S11.1BYBCh. 12 - Prob. S3.2BYBCh. 12 - Prob. 1TCCh. 12 - Prob. 2TCCh. 12 - In a genomic comparison between humans and yeast,...
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- Recombinant DNA Technology Provide a detailed explanation how DNA structure is exploited for the following techniques DNA Sequencing Molecular Cloning Polymerase Chain Reaction (PCR)arrow_forwardBacteria are frequently used in the generation of recombinant DNA molecules to: Group of answer choices A) Make copies of the vector + insert plasmid B) Ligate the insert into the vector C) Digest the vector for insert ligation D) Amplify the insert by PCRarrow_forwardDEFINE THE FOLLOWING: 1) restriction enzyme 2) plasmid 3) recombinant DNAarrow_forward
- What is recombinant DNA technology ?arrow_forwardb) Describe how DNA is digested by different restriction enzymes c) Describe how gel electrophoresis is used to estimate the size of DNA fragments.arrow_forwardFrom where do we get primers for sequencing DNA? A) they are synthesized by reverse transcriptase B) they are cut out of plasmids using restriction endonucleases C) DNA primase is added to the sequencing reaction and synthesizes the primers D) biotechnology companies synthesize them using organic chemistryarrow_forward
- Differentiate between plasmids, phage-based cloning vectors, cosmids, and artificial chromosomes in terms of structure and applicationarrow_forwardPCR has many useful applications. However, an incorrect application of PCR is: a) Amplification of any gene of interest from genomic DNA b) Screening various foods for genes that are typical in genetically modified organisms c) Identification of microbial DNA in a sample (viral or bacterial) d) Amplification of any protein of interestarrow_forwardWhat type of enzymes are used to “cut” desired DNA sequences for use in recombinant gene technology experiments? Identify those two enzymes used to cut and paste both genes into the plasmid. Identify all three strategies used in this lab to maximize transformation success. Explain what it means for bacteria to be “competent.” Explains why bacterial competency is this important for this investigation.arrow_forward
- In bacterial transformation, the purpose of having antibiotic within an agar plate is to: Select one: confirm which plasmids been have successfully ligated with a gene of interest. isolate bacteria which have been successfully transformed with the plasmid. indicate which plasmids were successfully digested by the endonuclease. act as a substrate which will be cleaved and produce a blue product when ligation is unsuccessful. show which plasmids contain the lacZ gene.arrow_forwardWrite in detail about the effects of presence as well as absence of multiple cloning site and reporter genes in a plasmid vector?arrow_forwardDescribe how the process of gene cloning results in a cell clonecontaining a recombinant plasmid.arrow_forward
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