Campbell Biology: Concepts & Connections (9th Edition)
9th Edition
ISBN: 9780134296012
Author: Martha R. Taylor, Eric J. Simon, Jean L. Dickey, Kelly A. Hogan, Jane B. Reece
Publisher: PEARSON
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Chapter 12, Problem 10TYK
A biologist isolated a gene from a human cell, inserted it into a plasmid, and inserted the plasmid into a bacterium. The bacterium made a new protein, but it was nothing like the protein normally produced in a human cell. Why? (Explain your answer.)
a. The bacterium had undergone transformation.
b. The gene did not have sticky ends.
c. The human gene contained introns.
d. The gene was not synthesized from scratch.
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Which of the following experimental results was NOT evidence that DNA is the genetic molecule rather than proteins?
a.
When a virus was radioactively labelled, and the virus was allowed to infect a bacteria cell, radioactive virus DNA was found inside the bacteria cell while radioactive virus proteins were found outside the cell.
b.
Dead pathogenic bacteria cells combined with living nonpathogenic bacteria cells caused the creation of living pathogenic cells and thus the death of the host animal.
c.
Proteins are a class of macromolecules with the diversity and specificity needed for hereditary material.
d.
Nucleotide bases occur regularly, such that the number of adenines = number of thymines, and the number of guanines = number of cytosines.
What is a restriction endonuclease?
Select one:
a. It is an enzyme that cleaves at a specific nucleotide sequence.
b. It restricts the movement of the DNA outside the nucleus.
c. It proofreads the DNA for accidental damages and corrects any errors.
d. It is an enzyme that separates the DNA double helix.
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Chapter 12 Solutions
Campbell Biology: Concepts & Connections (9th Edition)
Ch. 12 - Imagine you have found a small quantity of DNA....Ch. 12 - Which of the following would be considered a...Ch. 12 - The DNA profiles used as evidence in a murder...Ch. 12 - A paleontologist has recovered a tiny bit of...Ch. 12 - How many genes are there in a human sperm cell? a....Ch. 12 - When a typical restriction enzyme cuts a DNA...Ch. 12 - Why does DNA profiling rely on comparing specific...Ch. 12 - Recombinant DNA techniques are used to...Ch. 12 - A biochemist hopes to find a gene in human cells...Ch. 12 - A biologist isolated a gene from a human cell,...
Ch. 12 - Explain how you might engineer E. coli to produce...Ch. 12 - What is left for genetic researchers to do now...Ch. 12 - Today, it is fairly easy to make transgenic plants...Ch. 12 - In the not-too-distant future, gene therapy may be...Ch. 12 - The possibility of extensive genetic testing...Ch. 12 - SCIENTIFIC THINKING Scientists investigate...
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- Definition of Terms: a. Genetic Engineering b. DNA c. Recombinant DNA d. Plasmids e. Cloning f. Genome g. Gene Mapping h. Biotechnology i. Polymerase Chain Reaction j. Gene Therapyarrow_forwardWhich of the following describes sequencing by synthesis? A. Large sections of DNA are inserted into a plasmid. B. DNA sequence is recorded as the complementary strand is built. C. Many copies of the DNA are made, each terminating at a specific nucleotide. D. A DNA strand is forced through a pore and its sequence synthesized as it passes through.arrow_forwardExplain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.arrow_forward
- A researcher is performing PCR to amplify a sample of DNA. Unfortunately, he forgot to add the DNA primer prior to starting the experiment. Which of the following results is he most likely to observe? a. The reaction will work, but at a significantly slower rate. b. The reaction will work, but the product will contain many undesired mutations. c. The reaction will work, but amplify a region that was not his target. d. The reaction will be completely unsuccessfuarrow_forwardWhich of the following statements about DNA probes are false? a. Probes can be labeled with a fluorescent dye to facilitate detection. b. Probes can be labeled with radioactive phosphorus to facilitate detection. c. Probes are typically composed of double stranded pieces of DNA. d. Probes are typically composed of single stranded pieces of DNA.arrow_forwardA.) There is no change in the DNA sequence if nucleotides are added or removed, it will have no effect to the cell. B.) Mutations in the DNA sequence are all irreversible. A. Statement A is correct B. Statement B is correct C. Both A and B are correct D. Both A and B are incorrectarrow_forward
- Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forwardWhich of the following objects would you use microinjection in order to transfer DNA into? a. Egg cell visible to the human eye b. Animal virions c. Escherichia coli that has an F plasmid d. Bacteria that has been heat shockedarrow_forwardA company that manufactures cleansing products has synthesized two new organic compounds , chemicals A and B, which improve the cleaning power of dishwashing detergents. To determine the mutagenic capacity of the chemicals , the company tested the effect of the chemicals on Salmonella cells requiring histidine to grow ( His- cells ). The table below shows the results of the test . A. Name the test used by the company . B. What does the test detect or estimates ? C. Which chemical (s) would you identify as containing a mutagen ? Why ? D. What is the purpose of test 1 ? Why are colonies detected in test 1? E. Which chemical ( s) would you identify as possible antimutagen ? Why ?arrow_forward
- Which of the following describes an advantage of using a recombinant plasmid for DNA cloning over PCR? A. PCR is more likely to have errors introduced in the copying process. B. Recombinant DNA plasmids are able to create large amounts of copies more quickly than PCR. C. PCR can only be conducted in eukaryotic cells. D. PCR requires prior knowledge of the sequence in question, while a recombinant plasmid does not.arrow_forwardWhat is a cloning vector? A. The DNA probe used to locate a particular gene in the genome. B. An agent such as plasmid, used to transfer DNA from an in vitro solution into a living cell. C. The laboratory apparatus used to clone genes. D. An enzyme that cuts DNA into restriction fragments.arrow_forwardA.) Chemical changes to the DNA caused by mutations can cause hazardous damage to the DNA. B.) Environmental factors such as radiation can cause damage to the DNA of the cells. a. Statement A is correct b. Statement B is correct c. Both A and B are correct d. Both A and B are incorrectarrow_forward
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