Microbiology With Diseases By Taxonomy (6th Edition)
6th Edition
ISBN: 9780134832302
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 11, Problem 7SA
How is Agrobacterium used in recombinant DNA technology?
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Why are bacteria excellent hosts for recombinant DNA experiments?
Describe how would you determine the following using a real-time PCR instrument.
A) The quantity and quality of a DNA sample.
B) Whether an individual had cystic fibrosis from a genomic DNA sample.
C) If several bacterial pathogens were present in DNA extracted from a food sample.
Why is it important to use a hyperthermophilic DNA polymerase in PCR?
a) Because only hyperthermophiles have DNA polymerases.
b) Because hyperthermophilic DNA polymerase is able to resist the saline reaction conditions.
c) Because hyperthermophilic DNA polymerase is faster than other polymerases.
d) Because hyperthermophilic DNA polymerase is able to resist denaturation at 95℃.
Chapter 11 Solutions
Microbiology With Diseases By Taxonomy (6th Edition)
Ch. 11 - Prob. 1TMWCh. 11 - Why are taxonomic names and categories in our...Ch. 11 - Prob. 3TMWCh. 11 - Prob. 4TMWCh. 11 - Prob. 1MTFCh. 11 - Prob. 2MTFCh. 11 - Prob. 3MTFCh. 11 - Prob. 4MTFCh. 11 - Prob. 5MTFCh. 11 - Prob. 6MTF
Ch. 11 - Prob. 7MTFCh. 11 - Prob. 8MTFCh. 11 - Prob. 9MTFCh. 11 - Prob. 10MTFCh. 11 - Prob. 1MCCh. 11 - Prob. 2MCCh. 11 - Prob. 3MCCh. 11 - Prob. 4MCCh. 11 - How is Halobacterium salinarum distinctive? a. It...Ch. 11 - Prob. 6MCCh. 11 - Prob. 7MCCh. 11 - Prob. 8MCCh. 11 - Prob. 9MCCh. 11 - Prob. 10MCCh. 11 - Whereas the first edition of Bergeys Manual relied...Ch. 11 - What are extremophiles? Describe two kinds, and...Ch. 11 - Prob. 3SACh. 11 - Prob. 4SACh. 11 - Prob. 5SACh. 11 - Prob. 6SACh. 11 - How is Agrobacterium used in recombinant DNA...Ch. 11 - Name and describe five distinct classes of phylum...Ch. 11 - Label the shapes of these prokaryotic cells.Ch. 11 - Prob. 2VICh. 11 - Prob. 1CTCh. 11 - Prob. 2CTCh. 11 - Why is it that Gram-positive species dont have...Ch. 11 - Prob. 4CTCh. 11 - Prob. 5CTCh. 11 - Prob. 6CTCh. 11 - Prob. 7CTCh. 11 - Prob. 8CTCh. 11 - Prob. 9CTCh. 11 - Prob. 10CTCh. 11 - Prob. 11CT
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- Transfection efficiency refers to a) total number of transfected cells per dish O b) total number of transfected cells per field of view total amount of plasmid DNA added per sample d) number of cells per 100 that have taken up plasmid DNAarrow_forwardWhat is recombinant DNA technology ?arrow_forwardWhy is Agrobacteriummediated genetic engineering transformation inplants considered as natural genetic engineering?arrow_forward
- Which is used for cloning eukaryotic genes but not prokaryotic genes?a) Restriction enzymesb) DNA ligasec) Reverse transcriptased) Vectore) Selectable markerarrow_forwardWhy is PCR beneficial?arrow_forwardAn example of bioremediation is A) the use of prokaryotes to treat sewage or clean up oil spills. B) the production of antibiotics by cultured prokaryotes. C) the application of bacteria to produce transgenic plants.arrow_forward
- How are we to know which of the bacteria have taken up the plasmid?arrow_forwardSequence of which of the following cannot be determined using the Maxam Gilbert method?a) Bacteriab) Plantsc) Bacteriophage T7d) Plasmidarrow_forwardWhy do we use an Electron Scanning Microscope to see bacteriophages over other microscopes?arrow_forward
- 1. What is a plasmid? A) An organelle found in plants that conducts photosynthesis. B) Circular extrachromosomal DNA. C) An essential part of the bacterial chromosome. 2. What is the role of the NaOH in plasmid isolation? A) It increases the pH. B) It denatures the DNA. C) Both of the above. 3. In which direction will the DNA migrate in an agarose gel? A) To the positive pole. B) To the negative pole. C) That depends on the sequence of the DNA. 4. What is the function of SDS? A) It denatures proteins. B) It disintegrates the cell membrane. C) Both of the above. 5. To which species group does E. coli belong to? A) Bacteria, B) Fungi. C) Archaea.arrow_forwardWhy can’t Agrobacterium be used for transforming all plants?arrow_forward17) What gives Aspergillus sydowii colonies their hairy appearance? a) Hyphae () b) Stipes c) Growing media ) d) Ascocarpoda Jack has designed one primer which is complementary to the DNA of E. coli. He then used that primer for a PCR reaction using DNA isolated from a mixture of bacteria. He got no PC reaction product and hence concluded that there is absolutely no E. coli in the bacteria mixture. His conclusion is .... () True ) Falsearrow_forward
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