Prescott's Microbiology
Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 10.4, Problem 4RIA

In general terms, how is ΔG°′ related to ΔE0? What is the ΔE0 when electrons flow from the NAD+/NADH redox pair to the Fe3+/Fe2+ redox pair? How does this compare to the ΔE0 when electrons flow from the Fe3+/Fe2+ conjugate redox pair to the 1 2 O 2 / H 2 O pair? Which will yield the largest amount of free energy to the cell?

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Compare the delta ΔG0' values for the oxidation of succinate by NAD+ and by FAD. Use the data given in Table 18.1 to find the E0' of the NAD+-NADH and fumarate-succinate couples, and assume that E0' for the FAD – FADH2 redox couple is nearly 0.05 V. Why is FAD rather than  NAD+ the electron acceptor in the reaction catalyzed by succinate dehydrogenase?
With the help of the half reactions given in Table 1, formulate the redox equation for the oxidation of succinate and reduction of ubiquinone.
It is estimated that each electron pair donated by NADH leads to the synthesis of approximately three ATP molecules, whereas each electron pair donated by FADH2 leads to the synthesis of approximately two ATP molecules. What is the underlying reason for the difference in yield for electrons donated by FADH2 versus NADH?

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