EBK CONCEPTS OF GENETICS
12th Edition
ISBN: 9780134818979
Author: Killian
Publisher: YUZU
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Chapter 10, Problem 33ESP
During gel electrophoresis, DNA molecules can easily be separated according to size because all DNA molecules have the same charge-to-mass ratio and the same shape (long rod). Would you expect RNA molecules to behave in the same manner as DNA during gel electrophoresis? Why or why not?
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During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied?
because DNA is negatively charged
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because DNA is positively charged
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Chapter 10 Solutions
EBK CONCEPTS OF GENETICS
Ch. 10 - Would an experiment similar to that performed by...Ch. 10 - In sea urchin DNA, which is double stranded, 17.5...Ch. 10 - German measles results from an infection of the...Ch. 10 - What vital clues were provided by Franklins work...Ch. 10 - Was it ethical for Wilkins to show Franklins...Ch. 10 - Prob. 3CSCh. 10 - HOW DO WE KNOW? In this chapter, we first focused...Ch. 10 - CONCEPT QUESTION Review the Chapter Concepts list...Ch. 10 - Discuss the reasons proteins were generally...Ch. 10 - Contrast the contributions made to an...
Ch. 10 - When Avery and his colleagues had obtained what...Ch. 10 - Why were 32P and 35S chosen for use in the...Ch. 10 - Does the design of the HersheyChase experiment...Ch. 10 - What observations are consistent with the...Ch. 10 - What are the exceptions to the general rule that...Ch. 10 - Draw the chemical structure of the three...Ch. 10 - How are the carbon and nitrogen atoms of the...Ch. 10 - Adenine may also be named 6-amino purine. How...Ch. 10 - Draw the chemical structure of a dinucleotide...Ch. 10 - Describe the various characteristics of the...Ch. 10 - What evidence did Watson and Crick have at their...Ch. 10 - What might Watson and Crick have concluded had...Ch. 10 - How do covalent bonds differ from hydrogen bonds?...Ch. 10 - List three main differences between DNA and RNA.Ch. 10 - What are the three major types of RNA molecules?...Ch. 10 - How is the absorption of ultraviolet light by DNA...Ch. 10 - What is the physical state of DNA after it is...Ch. 10 - What is the hyperchromic effect? How is it...Ch. 10 - Why is Tm related to base composition?Ch. 10 - What is the chemical basis of molecular...Ch. 10 - What did the WatsonCrick model suggest about the...Ch. 10 - A genetics student was asked to draw the chemical...Ch. 10 - Considering the information in this chapter on B-...Ch. 10 - One of the most common spontaneous lesions that...Ch. 10 - In some organisms, cytosine is methylated at...Ch. 10 - Because of its rapid turnaround time, fluorescent...Ch. 10 - Prob. 31ESPCh. 10 - Newsdate: March 1, 2030. A unique creature has...Ch. 10 - During gel electrophoresis, DNA molecules can...Ch. 10 - DNA and RNA are chemically very similar but are...Ch. 10 - Electrophoresis is an extremely useful procedure...
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- in the gel than a A DNA molecule that is 1000 base pairs in length would go molecule that is 200 base pairs in length. A) further B) you cannot tell from the information provided C) the same distance D) not as fararrow_forwardPut the following pieces of DNA in order that they would appear reading from the top (nearest the loading point) to the bottom of a gel, with "first" meaning nearest the top. First [ Choqse [Choose] 21,000 basepairs (bp) Second 10,000 bp 23 kilobases (kB) 18 kB 2,300 bp Third [Choose ] Fourth [ Choose] [Choose] Fifth > >arrow_forwardWhen proteins are separated using native gel electrophoresis, size, shape, and charge control their rate of migration on the gel. Why does DNA separate based on size, and why do we not worry much about shape or charge?arrow_forward
- Which of the following correctly describes a possible scenario during a run of gel electrophoresis with DNA samples? Because DNA molecules are positively-charged due to the phosphate groups in their backbone, DNA fragements will migrate from the anode to the cathode. Because DNA molecules are negatively-charged due to the phosphate groups in their backbone, DNA fragments will migrate from the anode to the cathode. O Shorter fragments of DNA are lighter. Hence, they move faster through the gel and travel the farthest from the wells. Larger fragments of DNA move faster through the gel and travel the farthest from the wells.arrow_forwardYou study actin polymerization by monitoring the increase in fluorescence of pyrene-labeled actin. Pyrene is a fluorescent molecule, which has very low level of fluorescence on its own or when fused to G-actin. However, the pyrene fluorescence increases dramatically when pyrene-labeled G-actin is incorporated into a polymer. In this assay, the amount of pyrene fluorescence is proportional to the amount of actin polymer. In the figure below, actin polymerization curves resulted for conditions listed below; assume that G-actin concentration is above the critical concentration for the pointed end and that ATP is present. Which curve corresponds to which condition? Explainarrow_forwardIn DNA and plasmid extraction using the kit method, silica membrane is used. How could the nucleic acid attach to the membrane? O by phosphodiester bonding to the silica matrix O by hydrogen bonding in high salt conditions O by covalent bonding between nucleic acid and silica O by hydrophobic bonding in high chaotrophic salt conditionsarrow_forward
- In gel electrophoresis: O a) DNA, which is negatively charged, will travel from the anode (+) to the cathode (-) O b) DNA, which is negatively charged, will travel from the cathode (-) to the anode (+) O C) DNA, which is positively charged, will travel from the anode (+) to the cathode (-) O a) DNA, which is positively charged, will travel from the cathode (-) to the anode (+)arrow_forwardSuppose, you have a solution of DNA, RNA and some proteins. How can you separate the proteins from your sample solution with high efficiency? Describe the process.arrow_forwardHow are DNA molecules visualized in a gel after electrophoresis? Why do DNA molecules migrate toward the + electrode? What determines the rate of their migration? What is the effect of PEG on DNA fragments of different sizes? How is this influenced by the concentration of PEG?arrow_forward
- Why do we need to amplify the DNA at all in order to visualize it in a gel?arrow_forwardThe competency of bacterial cells to take up plasmids from the environment can be enhanced by treating them with calcium chloride. Which of the following statements is true regarding this process? Question 23 options: Chloride ions neutralize charges on the phospholipids and DNA. Chloride ions adhere to the cell membrane and calcium ions to the plasmid DNA, thus increasing the attractive force between them. The calcium ions change the structure of the cell membrane and, as a result, the pores are enlarged. Calcium ions neutralize charges on the phospholipids of the bacterial cell membrane and on the DNA of the plasmid. Chloride ions enter the cell through protein pores in the membrane, carrying plasmid DNA with them.arrow_forwardYou're purifying some plasmid DNA from a culture of bacteria and you want to know how pure it is. You measure the optical density at 260 nm and 280 nm and find the ratio is 2.0. You suspect there is RNA contamination in your preparation, so you treat your preparation with RNase. But the ratio is still 2.0. Protein assays tell you there is no protein in your solution, and no other biological molecules absorb light very efficiently at those wavelengths. What's the explanation?arrow_forward
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