SAPLINGPLUS F/BIOCHEM+ICLICKER REEF-CODE
9th Edition
ISBN: 9781319398583
Author: BERG
Publisher: MAC HIGHER
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 1, Problem 31P
Interpretation Introduction
Interpretation:
The energy of the ionic interaction between two phosphate groups at the given values of dielectric constant should be calculated.
Concept introduction:
Under the effect of electric field, the induced polarization gets involved in the volume of the given material. The measurement of the potential energy in the form of induced polarization is termed as dielectric constant. Further, dielectric constant is calculated as the ratio of dielectric permittivity of material to that of the vacuum.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
Protein,nfolded Proteinţolded
Under certain conditions, a specific protein has the following thermodynamic parameters for going from
the unfolded state to the folded state: AH = – 300 kJ/mol, and TAS = – 400 kJ/mol. Under those
conditions, the equilibrium would lie more toward
neither would predominate - the fraction of total protein that's folded would = fraction unfolded.
the folded state
the unfolded state
O It is impossible to predict the position of the equilibrium from AH and TAS.
Previous Assignment:
The figure below shows the bonding of the cytosine and guanine molecules. The O - H and H - N distances are each 0.110 nm. In this case, assume that the bonding is due only to the forces along the O - H - O, N - H - N and O - H - N combinations, and assume also that these three combinations are parallel to each other. Calculate the net force that cytosine exerts on guanine due to the preceding three combinations. Is this force attractive or repulsive?
----------------------------------------------------------------------------------
Electric Potential Energy: Base Pairing in DNA, IICalculate the electric potential energy of the guanine–cytosine bond, using the same combinations of molecules (O - H - O, N - H - N and O - H - N) as in the previous assignment (pls refer above)
Abosrbance (A 280)
You are interested in isolating an enzyme Ribonuclease. Inside cells ribonuclease self-associates and can
exist as dimers or trimers.
The active form of the enzyme is monomeric with molecular weight of ~ 14Kda.
In order to isolate ribonuclease, you use size exclusion chromatography column and obtain three peaks.
Indicate which peak (starting from left to right) corresponds to Monomer (M), Dimer (D) and Trimer (T)
forms of ribonuclease.
PEAK I:
PEAK II:
PEAK III:
Time
M
м
Fractions
Chapter 1 Solutions
SAPLINGPLUS F/BIOCHEM+ICLICKER REEF-CODE
Ch. 1 - Prob. 1PCh. 1 - Prob. 2PCh. 1 - Prob. 3PCh. 1 - Prob. 4PCh. 1 - Prob. 5PCh. 1 - Prob. 6PCh. 1 - Prob. 7PCh. 1 - Prob. 8PCh. 1 - Prob. 9PCh. 1 - Prob. 10P
Ch. 1 - Prob. 11PCh. 1 - Prob. 12PCh. 1 - Prob. 13PCh. 1 - Prob. 14PCh. 1 - Prob. 15PCh. 1 - Prob. 16PCh. 1 - Prob. 17PCh. 1 - Prob. 18PCh. 1 - Prob. 19PCh. 1 - Prob. 20PCh. 1 - Prob. 21PCh. 1 - Prob. 22PCh. 1 - Prob. 23PCh. 1 - Prob. 24PCh. 1 - Prob. 25PCh. 1 - Prob. 26PCh. 1 - Prob. 27PCh. 1 - Prob. 28PCh. 1 - Prob. 29PCh. 1 - Prob. 30PCh. 1 - Prob. 31PCh. 1 - Prob. 32PCh. 1 - Prob. 33PCh. 1 - Prob. 34PCh. 1 - Prob. 35PCh. 1 - Prob. 36P
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.Similar questions
- . Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pk, = 6.0) when the other has a valine (uncharged side chain). (a) Which would be better for separation: gel electrophoresis or isoelectric focusing? Why? (b) What pH would you choose for the separation?arrow_forwardAla-Arg-Val-His-Asp-Gln Given the polypeptide chain above Estimate the net charge of the polypeptide chain at physiological pH (7.4) and at pH 5.0 . How many peptide bonds are there? What kind of polypeptide is it?arrow_forwardDNA has pKa ≈ 1.0. At normal pH what would the charge be for a double helix that is chain 50 angstroms long?arrow_forward
- Draw Haworth projections formulas for dimers of glucose with the following glycosidic linkages: 1. An alpha (1-4) linkage 2. A beta (1-6) linkagearrow_forwardComponents of Complex Biomolecules Figure 1-10 (Lehninger's Biochemistry book) shows the major components of complex biomolecules. For each of the two important biomolecules below (shown in their ionized forms at physiological pH), identify the constituents. And show H-bonding with water. (a) Guanosine triphosphate (GTP), an energy-rich nucleotide that serves as a precursor to RNA: N- NH -CH2 0- NH2 H H H H. ОН ОН (b) Phosphatidylcholine, a component of many membranes: CH3 O- CH3*N-CH2-CH2-0-P-0–CH2 нн CH3 НС —О—С—(CH), —С—С— (CH), — СHз CH,–0-C-(CH,)14–CH3arrow_forward2. Subunit Composition of a Protein. A protein has a molecular mass of 400 kDa when measured by size-exclusion chromatography ( F). When subjected to gel electrophoresis in the presence of sodium dodecyl sulfate (SDS), the protein gives three bands with molecular masses of 180, 160, and 60 kDa. When electrophoresis is carried out in the presence of SDS and dithiothreitol (=), three bands are again formed, this time with molecular masses of 160, 90, and 60 kDa. Determine the subunit composition of the protein. Note: dithiothreitol is a reagent to beak down the disulfide bonds.arrow_forward
- Suppose you have a solution of a protein, which contains a specific Tyr residue that has an actual (measured) pKa of 8.8. The protein binds a ligand by several noncovalent interactions, one of which is a hydrogen bond in which the Tyr phenolic hydroxyl group must serve as a hydrogen bond donor. Calculate the percentage of the protein molecules in which that tyrosyl residue's phenolic hydroxyl group could serve as a hydrogen bond donor at pH 8.5arrow_forwardE Threonine 6. You have identified some intermediates in threonine synthesis: A, B, C, D and E. You grow a few of your mutants in the presence of these different intermediates to determine the order in which the gene products act. Below are your results. A (+) means growth and a (-) means no growth. Given these data, draw the best possible pathway for the synthesis of threonine. The diagram should use arrows to indicate one intermediate being changed to another intermediate. Indicate which gene produces the product responsible for the conversion by listing the mutant in that gene above the arrow. Mt1 Mt2 Mt4 Mt7arrow_forwardThe melting curve for the polyribonucleotide poly(A) is shown below. (a) Explain why absorbance increases with increasing temperature. (b) Why does the shape of the curve differ from the one shown in Fig. ?arrow_forward
- Suppose two phosphate groups in DNA (each with a charge of 2 1) are separated by 12 Å. What is the energy of the ionic interaction between these two phosphates assuming a dielectric constant of 80? Repeat the calculation assuming a dielectric constant of 2.arrow_forwardCyclic forms of D-glucose: Haworth formula The furanose and pyranose forms of D-glucose can be presented using Haworth formula. The following templates are used for this formula. Note the numbering of the carbon atoms and the anomeric carbon (this carbon defines the alpha and beta isomer). Furanose template (furan) Pyranose template (pyran) anomeric carbon anomeric carbon Compare the Fisher and Haworth formula of D-glucofuranose and D-glucopyranose. Notice that the groups attached to C-1, C-2, and C-3 on the left side of the Fisher formula are written above the plane of the furanose template. Fisher formula Haworth formula H FOH H-2 HOH,č 5 -OH FOH- H HO-3H 4 он H 1 H-4 H-5 H OH 3 6 CH2OH H OH Alpha-D-glucofuranose Alpha-D-glucofuranose HOH2C- он OH но H FOH- OH Но H H. OH H ČH2OH Beta-D-glucofuranose Beta-D-glucofuranosearrow_forwardDenaturation of Proteins The following are four levels of protein structures. Identify the inter- and intramolecular forces of attractions that stabilized each level. Level of Protein Structure Forces of Attraction Present Primary Secondary Tertiary Quaternary 2.) What is denaturation? 3.) What are denaturing agents? Give three examples and describe their effects on protein. 4.) Differentiate reversible and irreversible denaturation. 5.)Why is 70% ethanol is more effective than 100% ethanol to kill bacteria?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- BiochemistryBiochemistryISBN:9781319114671Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.Publisher:W. H. FreemanLehninger Principles of BiochemistryBiochemistryISBN:9781464126116Author:David L. Nelson, Michael M. CoxPublisher:W. H. FreemanFundamentals of Biochemistry: Life at the Molecul...BiochemistryISBN:9781118918401Author:Donald Voet, Judith G. Voet, Charlotte W. PrattPublisher:WILEY
- BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage LearningBiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage LearningFundamentals of General, Organic, and Biological ...BiochemistryISBN:9780134015187Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. PetersonPublisher:PEARSON
Biochemistry
Biochemistry
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:9781464126116
Author:David L. Nelson, Michael M. Cox
Publisher:W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul...
Biochemistry
ISBN:9781118918401
Author:Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:WILEY
Biochemistry
Biochemistry
ISBN:9781305961135
Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:Cengage Learning
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Fundamentals of General, Organic, and Biological ...
Biochemistry
ISBN:9780134015187
Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:PEARSON
Biomolecules - Protein - Amino acids; Author: Tutorials Point (India) Ltd.;https://www.youtube.com/watch?v=ySNVPDHJ0ek;License: Standard YouTube License, CC-BY