1.4 Transition breadth 1.3 1.2 1.1 1.0 50 70 90 Temperature (°C) Relative absorbance at 260 nm 20 40 60 80 100 Temperature (°C) Relative absorbance at 258 nm

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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The melting curve for the polyribonucleotide poly(A) is shown below. (a) Explain why absorbance increases with increasing temperature. (b) Why does the shape of the curve differ from the one shown in Fig. ?

1.4
Transition breadth
1.3
1.2
1.1
1.0
50
70
90
Temperature (°C)
Relative absorbance at 260 nm
Transcribed Image Text:1.4 Transition breadth 1.3 1.2 1.1 1.0 50 70 90 Temperature (°C) Relative absorbance at 260 nm
20
40
60
80
100
Temperature (°C)
Relative absorbance at 258 nm
Transcribed Image Text:20 40 60 80 100 Temperature (°C) Relative absorbance at 258 nm
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