You want to replicate Francesco Redi’s “rotting meat” experiment in disproving spontaneous generation, but improve upon the original experimental design. You have access to: ●1 kg of uncooked minced beef ●Gas cook top ●Fry pan ●Plastic cling wrap ●Bowls ●Refrigerator set to 4 degrees 1A. Describe TWO (2) experimental variables you will change and monitor, and how you will use the above items to do so.
Q: The receptor or drug target in this case was the rat GABAA receptor obtained from homogenised rat…
A: The primary elements of any signaling pathway are the ligand and receptors. The ability of a ligand…
Q: Which of the following nutrients in food are most efficiently metabolized by microflora of ruminant…
A: The microflora of ruminant animals refers to the complex community of microorganisms, including…
Q: 7. A boy born with colour blindness has a dad who is colour blind but his mom is not. He is most…
A: Color blindness is often an X-linked recessive trait. This means that the gene responsible for color…
Q: Blood is a crucial component of the cardiovascular system. Beside each job, write which type of…
A: White blood cells (WBCs) particularly neutrophils play a crucial role in the immune response. They…
Q: Which of the following statements applies to non-sister chromatids? they are genetically identical…
A: The correct statement that applies to non-sister chromatids is:C.They contain the same genes in the…
Q: If you run IHC and the antibody you chose binds to everything in a non-specific manner, what visual…
A: Immunohistochemistry (IHC) could be a commonly utilized strategy in science and medication to see…
Q: In an ant colony in which the queen is mated with 2 males, the average relatedness of a female…
A: The proportion of worker-produced males, the number of related queens, and the projected worker-male…
Q: During this week you have learned about the muscular and the skeletal systems. Many athletes have…
A: As per our guildlines this is not a type of questionYou haven't provide the information about…
Q: What is one way that cancer cells typically avoid being detected and targeted by immune cells, such…
A: Cancer or tumor is formed by the repeated and uncontrolled cell division. Cancer cells rcome up with…
Q: The TRP operon is regulated by two mechanisms, the TrpR regulatory protein and attenuation.…
A: The TRP operon is a well-studied genetic system that plays a crucial role in regulating the…
Q: Molecular Biology-Transcription, Translation, Replication, Gene Action 1) What is transcription,…
A: Transcription refers to the process of copying genetic information from DNA to RNA, which is vital…
Q: Can someone please help with the graph and number 4? Thank you.
A: Population growth is the gradual rise in the total number of individuals within a population. It is…
Q: salamanders, green skin (G) is dominant yellow skin (g), a detachable tail (D) is dominant over a…
A: In this genetic cross involving salamanders, we are examining three different traits: skin color,…
Q: An aqueous solution of hydrochloric acid is standardized by titration with a 0.150 M solution of…
A: Ba(OH)2 (aq) +2 HBr (aq) = BaBr2(aq)+2H2O(l) At equivalence point : Number of moles of Ba(OH)2 =…
Q: As a general rule, ascorbic acid should be provided as a supplement to diets of all mammals but not…
A: Ascorbic acid, also known as vitamin C, is a water-soluble vitamin that is essential for human…
Q: The data below illustrates the role of MutS, MutH and Dam in DNA repair. Strains of E coli that are…
A: Answer .) :- MutS and MutH are two proteins involved in the DNA mismatch repair (MMR) pathway in E.…
Q: As electrons are passed through the system of membrane proteins in photosystem II and photosystem I,…
A: The three correct options are in next step
Q: True or False: In a eukaryotic cell, the organelles called peroxisomes serve the purpose of…
A: The smallest unit of a living entity, including you and other creatures, is a cell. The metabolic…
Q: Which of the following does NOT happen during translation initiation? The mRNA binds to the small…
A: Translation: It is provess via which the protein is synthesized by the ribosomes after tye process…
Q: biotechnology lab class : 1. The bacterial streaking on antibiotic containing agar plates has been…
A: Using restriction endonucleases, a type of enzyme, restriction digestion, also known as restriction…
Q: If the trait shown in the pedigree presented follows an X-linked mode of inheritance, what is the…
A: Pedigree: In terms of genetics, a pedigree is a diagram demonstrating the manner in which a trait or…
Q: Give only typing answer with explanation and conclusion 1. e coli divides every 20 mins. assuming…
A: Bacterial growth typically follows four distinct phases: the lag phase, log or exponential phase,…
Q: From a presumed testcross of 2 plants of genotype Aa x aa (in which A represents red and a…
A: A test cross is a breeding genetic experiment that is utilized to determine the alleles present in…
Q: Which statement is NOT consistent with our model of atomic structure. The electrons are located…
A: Atom is the basic unit of matter. The nucleus of the atom is composed of protons and neutrons, which…
Q: . Which of these land plants have a dominant sporophyte phase? Please select all correct answers.…
A: "Since you have posted multiple questions, we will provide you the solution of only first question…
Q: Gram negative cells contain cell membrane and envelope (pick all that apply) Select one or more: a.…
A: Gramme staining is a technique for classifying bacteria into two major groups: gram-positive and…
Q: The figure below summarizes the results of an experiment evaluating the role of Ku80 gene DNA…
A: The gene knockout is performed for the detection of the role of a specific gene. The elimination of…
Q: In a population of 1000 "selfing" individuals, the allele frequency of A1= 0.6. Determine the number…
A: Understanding the mechanisms of inheritance and evolution depends on the study of allele frequencies…
Q: Question 32 are periods of evolutionary change in which many new species evolve from a coma…
A: Evolution is the process by which new characteristics develop or change in characteristics of a…
Q: In the absence of this enzyme, a substance called ceroid lipofuscin accumulates in lysosomes in the…
A: Batten disease is also known as neuronal ceroid lipofuscinoses. Batten disease is an autosomal…
Q: Consider the following hypothetical gene a plant. Gene T produces a protein that impacts stem…
A: In this scenario, the gene T displays incomplete dominance, which means that the heterozygous…
Q: (d) Scientists believe that the yellow fern arose from hybridization with another fern on the…
A: Hybridization is common and considered as a critical developmental drive to extend intraspecific…
Q: Rate of photosynthesis mL hr Rate of photosynthesis(- Green light = Rate of volume change light (…
A: Photosynthesis is a metabolic process that converts water and carbon dioxide to glucose and oxygen…
Q: People often use the word theory to refer to a guess about something. In the scientific sense, the…
A: A well-supported explanation or framework that has withstood a great deal of testing and criticism…
Q: What is meant by “extreme pain, respiratory arrest (breathing stops), severe muscular contractions,…
A: Electric current levels ranging from 50 to 150 milliamperes (mA) represent the magnitude of the…
Q: Complex IV iron catalyzes the reduction of O2. copper cytochrome c oxidase ALL OF THESE are good…
A: Cytochrome c oxidase or Complex IV is a large transmembrane protein complex located in the inner…
Q: What is the allelic frequency of A for the following population: Genotype No. individuals AA 450 Aa…
A: Allele frequency is also known as gene frequency.It is the number of individual alleles of a…
Q: The only way to have blue eyes is: Group of answer choices to be homozygous recessive for blue…
A: Homozygous: If the genotype of an individual has two identical alleles (e.g RR or rr) then, it is…
Q: 5) The information codon codes for a. 3; amino acids; 1 d. 3; amino acids; 3 in the mRNA is…
A: The sequence of codons in mRNA determines the sequence of amino acids that will be incorporated into…
Q: After streaking microbial culture on agar plates and observing colonial growth, TMTC usually…
A: TMTC ("Too Many to Count") is a condition in which the number of colonies on an agar plate exceeds…
Q: A scientist observing a cell during gene expression would be able to easily distinguish it as a…
A: The process by which an RNA molecule is created from genetic information contained in DNA is called…
Q: Cucumbers and melons are both members of the genus Cucumis, which includes members with distinct…
A: In the study of cucumbers and melons, scientists aimed to enhance disease resistance in a new fruit…
Q: yruvate dehydrogenase phosphatase is the regulatory component of pyruvatedehydrogenase complex.…
A: Pyruvate dehydrogenase phosphatase (PDP) is an enzyme that regulates the activity of pyruvate…
Q: 3. Flip the coins 100 times and record your results in the chart below. Class results Your results…
A: 1- Here each person has one coin. And each coin can be considered as parent with the genotype Aa of…
Q: The process by which you get a different assortment of your 4 grandparents' genes (as compared to…
A: The scientific branch of genetics deals with study of genetic diversity, genes, and inheritance.…
Q: How does maternal effects, epigenetics inheritance and extranuclear inheritance deviate from…
A: Mendelian inheritance is a type of biological inheritance that follows the principles/laws…
Q: Which below would be ways to create an allohexaploid (6N)? Check all that are correct.…
A: Defect in chromosomal segregation during gammat formation will responsible for the production of…
Q: B. A woman is a carrier of a Robertsonian translocation between chromosomes 18 and 21. (i) Her…
A: The question is about a woman who has a rearrangement of her chromosomes 18 and 21 called…
Q: In a pedigree, a female is represented with a square while a male is represented with a circle. True…
A: The occurrence and emergence of a certain gene or organism, as well as its ancestors, from one…
Q: A person with blue eyes is called Group of answer choices homozygous dominant heterozygous…
A: The expression of the eye colour ranges from brown to blue and virtually anything in between . This…
Step by step
Solved in 3 steps with 3 images
- Look at the table below and answer the following questions: Culture 1/10 1/50 1/100 1/200 dilution OD Reading 1 2.0 0.36 0.18 0.11 OD Reading 2 1.9 0.35 0.18 0.08 OD Reading 3 1.8 0.33 0.16 0.09 1. Which 2 dilution levels of the culture are the best to use to compute for its optical density? 2. What is the average optical density of the culture? (use values from 2 dilution levels)A culture is incubated for 10 hours. 1 hours after inoculation it reached the exponential growth phase. At this time point the cell density was 1x10^4 cells/ml. 5 hours after inoculation (still during the exponential growth phase) the cell density was 1x10^7 cells. Calculate K and g(t). The growth constant (k) is minute. (round to 3 decimal points) The generation time (gt) is minutes. (round to whole number)volume of the quasi-steady-state culture was V0= 500 L, and the nutrient solution containing glucose was added at a constant flow rate of F = 50 L/h.Data: X0 (at the beginning of feeding) = 20 g/L, S0 = 300 g/L, max = 0.2 h-1, KS = 0.5 g/L and Y x/s= 0.3 g/g a) Determine the volume of the culture at t = 10hb) Determine the concentration of glucose at t = 10 hc) determine the concentration and total mass of cells at t = 10 hd) If product is associated with growth with α = 1.5 and P0 = 0.1 g/L, determine the concentration of product at t = 10h. (answer:P = 67,55 g/L)
- As shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the number of colony forming units (CFU) per mL it contains. You do a plate count with these growth results (no. of colonies for each dilution): 1:10 too many to count; 1:100 too many to count; 1:1,000 174; 1:10,000 23; 1:100,000 no growth. The number of CFU per mL in the original culture was: 1 ml 1 ml Original inoculum Dilutions 9 ml broth in each tube 1:10 1 ml 174,000 1:100 1 ml 1 ml 1 ml 1:1000 1 ml 1:10,000 1 ml None of the other four answers (Correct answer not given) 1,000 230,000 174 1 ml 1:100,000 1 mlAs shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the number of colony forming units (CFU) per mL it contains. You do a plate count with these growth results (no. of colonies for each dilution): 1:10 too many to count; 1:100 too many to count; 1:1,000 312; 1:10,000 38; 1:100,000 no growth. The number of CFU per mL in the original culture was: A. 38 B. 380,000 C. 38,000 D. None of the other four answers (Correct answer not given) E. 10,000Here is an image of a UV-irradiation (wavelength 254nm) experiment performed on a plate of E. coll after incubation. Which side of the plate do you think has a zero-minute' negative control? A or B or neither? Explain with proper reasoning.
- A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…
- Determine the CFU/ml of the original culture in the image below. Write your value in scientific notation using the "^" symbol to denote an exponent. (Example: 1x10^3). Write the number only (no labels, like CFU/ml). 100 μ. . A Original Culture 1.2x10^6 100 μ. 100 μ. Lawn of growth 100 μ. B΄900 ul c|900 μl | D |900 μ. € 900 μ. | 100 με 1100 με 1100 με 1100 μ. 1,198 120 colonies colonies O 00 10 coloniesThe number of bacteria in saliva samples was determined by collecting the saliva, making serial dilutions, and inoculating nutrient agar by the pour plate method. The plates were inoculated aerobically for 48 hours at 37°C. What can you conclude from this data? Why? Mouthwash CFU per ml in salvia Before Use After Use Listerine 2.22 x 107 1.22x107 Schmidts Mouthwash 2.42 x 107 3.22x105 Biotene Mouthwash 3.21 x 107 2.12 x106When testing the efficacy of an antibiotic against bacteria on an agar plate, it is important to spread the bacteria evenly across the plate before you add the antibiotic. Why do the bacteria need to be applied evenly? None of the following are true All of the following are true If you add more bacteria in some areas compared to others, it might obscure the effect of the antibiotic If the area right next to the antibiotic initially received relatively few bacterial cells, that might lead you to overestimate the effectiveness of the antibiotic If the area right next to the antibiotic initially received a relatively large number of bacterial cells, that might lead you to underestimate the effectiveness of the antibiotic