You want to produce mass amounts of oxytocin, a small peptide hormone produced by the pituitary gland in humans that plays a role in uterine contraction during child birth and lactation during nursing. Your basic approach will be to obtain the gene for oxytocin from the pituitary gland, splice it into a bacterial plasmid, then grow lots of the bacteria to obtain lots of the hormone. For each tool or item listed below, describe what role it plays in the approach you are using to make lots of oxytocin. Bacterial plasmid Restriction enzyme DNA ligasec DNA
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
You want to produce mass amounts of oxytocin, a small peptide hormone produced by the pituitary gland in humans that plays a role in uterine contraction during child birth and lactation during nursing. Your basic approach will be to obtain the gene for oxytocin from the pituitary gland, splice it into a bacterial plasmid, then grow lots of the bacteria to obtain lots of the hormone. For each tool or item listed below, describe what role it plays in the approach you are using to make lots of oxytocin.
Bacterial plasmid
Restriction enzyme
DNA ligasec
DNA
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