You have synthesized an enhancerless GFP reportergene in which the jellyfish GFP cDNA is placeddownstream of a basal promoter that functions inmice. You will now fuse this enhancerless reporter tothe three types of sequences listed below (x–z).a. Which of the three types of sequences would youuse for which of the three listed purposes (i–iii)? Ineach case, explain how the particular fusion wouldaddress the particular use.Types of sequences fused to the reporter:x. random mouse genome sequencesy. known mouse kidney-specific enhancerz. fragments of genomic DNA surroundingthe transcribed part of a mouse geneUses:i. to identify a gene’s enhancer(s)ii. to express GFP tissue-specificallyiii. to identify genes expressed in neuronsb. Which of the sequences (x–z) would you fuse to aparticular mouse gene of interest in order to express the protein product of the gene ectopically,that is, in a tissue in which the gene is not usuallyexpressed? Why might you want to do this experiment in the first place?
You have synthesized an enhancerless GFP reporter
gene in which the jellyfish GFP cDNA is placed
downstream of a basal promoter that functions in
mice. You will now fuse this enhancerless reporter to
the three types of sequences listed below (x–z).
a. Which of the three types of sequences would you
use for which of the three listed purposes (i–iii)? In
each case, explain how the particular fusion would
address the particular use.
Types of sequences fused to the reporter:
x. random mouse genome sequences
y. known mouse kidney-specific enhancer
z. fragments of genomic DNA surrounding
the transcribed part of a mouse gene
Uses:
i. to identify a gene’s enhancer(s)
ii. to express GFP tissue-specifically
iii. to identify genes expressed in neurons
b. Which of the sequences (x–z) would you fuse to a
particular mouse gene of interest in order to express the protein product of the gene ectopically,
that is, in a tissue in which the gene is not usually
expressed? Why might you want to do this experiment in the first place?
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