You have isolated a Gram positive cocci from a throat culture. You have determined that it is catalase negative and is beta hemolytic. What test should you perform next to help identify it?
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- With your tests you now figured out that your patient has a Staphylococcus infection, and you would like to know if the infection is caused by Staphylococcus aureus or by Staphylococcus epidermis. What test can you do next to see if your patient indeed has a Staphylococcus aureus infection? O You can isolate the pathogen from the patient and grow it on a mannitol salt agar. Staphylococcus aureus can ferment mannitol turning the agar plate yellow. Staphylococcus erpidermis cannot ferment mannitol and the agar plate stays red. O You can isolate the pathogen from the patient and grow it on a mannitol salt agar. Staphylococcus epidermis can ferment mannitol turning the agar plate yellow. Staphylococcus aureus cannot ferment mannitol and the agar plate stays red. O There is no way you can distinguish between Staphylococcus aureus and Staphylococcus erpidermis O you do a Gram stain. Staphylococcus aureus will stay purple as it is Gram positive and Staphylococcus erpidermis will show up as…You want to isolate staphylococci from a clinical sample, as you suspect the patient has an infection with Staphylococcus aureus. What media would best isolate staphylococci from the other organisms in the culture?in the clinic, the acid-fast stain is used to diagnose infection with which bacterium?
- You want to identify whether a patient in the hospital has an infection caused by Staphylococci or Streptococci. How could you go about figuring this out? O You do a catalase test as most Stapholycoccus are catalase positive and most Streptococcus is catalase negative. O You do a Gram stain and check if the cell structure is a grape cluster of cocci or a string of cocci/ pairs of cocci. O all of the aboveYou are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies.a) What was the dilution factor?b) How many bacteria were present in the soil?2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells.a) After 5 hours, how many generations have occurredb) After 5 hours, how many bacteria are present?3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?you are given a mix culture of S aureus, E. coli and P. aeruginosa. besides using the streak plate method what other method could you choose to separate the bacteria? the result you will be looking for and interprete the result.
- Abigail did not dry the top of her agar plate and stored it top up. Now she wants to count colonies but there is a smear over the entire plate. She was working with E. coli. Why does she have a smear?If an unknown bacteria was given that was gram-positive coccus, and the catalase test was performed, what can be determined from the result of the test?After the original specimen has been plated and a reported gram-negative rod was seen on the initial gram stain, what is your initial assumption of the organism causing this disease?
- The smear prepared from the gram-positive control shown gram-negative results. Describe 3 reasons why this incorrect reaction could occur Can the possession of flagella by bacteria be utilized for classification purposes? Explain.a) explain in your own words how to do a nitrate reduction test to identify unknown bacteria b) explain in your own words how to perform a phenol red broth (prb) test to help identify unknown bacteriaYou have identified two colony types A and B on the streak plate. Now briefly describe (in 3-4 sentences) the process of how you identified the cellular morphology/arrangement of each isolate. Again, assume you have all the necessary equipment and materials at your disposal. Be concise and thorough, not verbose; e.g., refer to the Gram stain, but describing the details of each step is not necessary.