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What's the difference between surface plasmon resonance spectroscopy and surface plasmon resonance microscopy?
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- What advantage do fluorescent dyes and fluorescence microscopy provide in comparison to the chemical dyes used to stain specimens for light microscopy?What is phase contrast? Give examples of phase contrast in optical microscopy.What is the advantage of phase-contrast microscopyover bright-field microscopy?
- a) Describe the technique Of Atomic Force Microscopy under the following headings: (i) Basic principle of AFM and overview of operations (ii) Modes of A FMExplain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?Why is methylene blue used to stain cells? What does it bind to? Which type of microscopy should you use to visualize cells stained with methylene blue?