How is confocal scanning laser microscopy differentfrom fluorescence microscopy? In what ways are theysimilar?

Q: Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron,…

A: There are different types of microscopy like bright-field, phase-contrast, dark-field, fluorescence,…

Q: Why should the microscope be calibrated for each objective and prior to each use?

A: Introduction  Calibration is the process of establishing up an instrument such that it can produce a…

Q: Describe the basic plan of an optical microscope, and differentiatebetween magnification and…

A: The microscope is the major tool in the identification of a microorganism after its isolation. The…

Q: What does it mean when a microscope is said to be parfocal?

A: Microscope is an instrument which is used to magnify and resolve an image produce by an object which…

Q: Describe the basic plan of an optical microscope, and diff erentiate between magnifi cation and…

A: The things or objects that are not in the resolution  range of a normal eye or cannot be seen with…

Q: What particular features of fluorescence make it useful for microscopy?

A: Step 1 Microscopes are an instrument which is used for observing small objects and finer internal…

Q: What are the main characteristics of scanning electron microscopy?

A: Electrons are the negatively charged particles having minus 1 charge  and represented by e- ,…

Q: Describe the process of Scanning Confocal Microscopy?

A: Microscopy allows magnification of structures to visualize them.

Q: Would phase microscopy be of any particular value when examining a stained specimen? Explain

A: The area or field of study in which microscopes are used to view an object that cannot be seen…

Q: Why is CSLM able to view different layers in a thick preparationwhile bright-field microscopy…

A: The technical field that uses microscopes to observe objects and their areas that cannot be seen…

Q: Compare and contrast light microscopy and electron microscopy

A: Microscopy is the technical field of using microscopes to view objects and areas of objects that…

Q: What is the purpose of compound light microscope? What is a compound light microscope and where did…

A: A microscope is a device to observe objects that cannot be seen with naked eyes or viewing finer…

Q: discuss the microscopy technique that gives the best constrast image of non biological samples such…

A: Microscopy is an instrument used to magnify the size of the objects or particle which cannot be seen…

Q: a.  Why must you slowly turn the fine adjustment knob? b.  Is natural light or is artificial light…

A: The optical magnifying instrument, additionally alluded to as a light microscope is a sort of…

Q: What is the limit of resolution for a bright-field microscope?What defines this limit?

A: As a general  bright-field microscope, the  limit to magnification  is around 1300X and the limit of…

Q: List the optical parts of the microscope. How does it achieve magnification? Resolution?

A:

Q: outline the use, description, history, and functionality of a compound light microscope

A: Description of Compound Microscopes:-  A compound microscope is an upright microscope that uses two…

Q: Why should you always begin to use a microscope with a low-power objective?

A: Microscope is an instrument used to see the objects that cannot be seen by naked eye. A light…

Q: What is the difference between a phase-contrast and differential interference-contrast microscope?…

A: Microscope is an instrument that gives large images of small objects, This allows an exceedingly a…

Q: List two ways that a stereoscopic dissecting microscope differs from a compound microscope

A: The stereoscopic dissecting microscope is often used to study the surface of specimens. The compound…

Q: What is the purpose of confocal microscopy technique ?

A: The microscope instrument is used to view the objects, which are not visible under the naked eye.…

Q: What limitation applies to most forms of electron microscopy?

A: When we use Electron microscopy then we face certain limitations or drawbacks. Here we will discuss…

Q: why is microtome important in microscopy?

A: Objects or organisms that cannot be viewed by the unaided eyes are studied under the microscope.…

Q: What is the difference between a simple and a compound microscope?

A: A microscope refers to the laboratory instrument that is used to view tiny objects, which cannot be…

Q: What advantages do confocal and deconvolution microscopy provide in comparison to conventional…

A: Confocal microscopy uses light from laser, elimination of background information away from the focal…

Q: What are the advantages of using super-resolution microscopyversus standard fluorescence microscopy?

A: Fluorescence microscope is a type of microscope that makes use of phosphorescence and fluorescence…

Q: What is the difference between a scanning and a transmission electron microscope? When do you use…

A: Microscopes are the instruments that allow scientists to study objects that cannot be seen through…

Q: How do the different parts of microscope serve their specific function? Cite examples

A: Introduction Cell is the basic unit of life which perform all kind of physiological and metabolic…

Q: different components of microscope and functions?

A: Light microscope indicates any type of microscope that utilizes visible light to visualize…

Q: What is the advantage of phase-contrast microscopyover bright-field microscopy?

A: Phase-contrast microscopy is an optical microscopy technique that converts phase shifts in light…

Q: What major advantage does phase-contrast microscopy haveover staining?

A: Phase-contrast microscopy is an optical microscopy technique used in cells. When the light passes…

Q: What are the different lens of the microscope?

A: Microscope: It is a laboratory optical instrument that is used to examining object that are too…

Q: what is the source of illumination for a light microscope?

A: A microscope is an optical instrument used to view objects, for example, animal or plant cell, that…

Q: Explain the differences between Brightfield, Darkfield, and Phase-Contrast Microscopy.

A: * Here are some differences between bright field microscopy darkfield miccroscopy and phase contrast…

Q: Explain in detail simple and differential staining and also improving and adjusting contrast in…

A: Staining is a chemical method which is used to colour the organism or the cell so that it is viewed…

Q: How many types of objective lenses a light microscope has?

A: In a microscope, the objective lens lies very close to the specimen. The objective lens collects the…

Q: Describe the use of fluorescent dyes in microscopy.

A: Microscopy is a technical field that involves utilizing microscopes to examine materials and things…

Q: Why is methylene blue used to stain cells? What does it bind to?

A: Answer: Methyl blue is used to stain the cell because it is basic dye which can easily bind with…

Q: What advantage do fluorescent dyes and fluorescence microscopy provide in comparison to the chemical…

A: Microscope is the widely used laboratory instrument in the molecular biology, it is mainly used to…

Q: microscopy, why are different stains used for different samples? Be specific.

A: A microscope is an instrument which is used to observe microscopic specimens which are otherwise not…

Q: what are two advantages and disadvantages of electron microscopes?

A: Introduction: An electron microscope is a microscope that uses accelerated electrons as a source of…

Q: What is the principle of image formation on transmission election microscope and scanning electron…

A: The general biological microscope helps in the visualisation of the image of a particular sample…

Q: How does differential interference contrastmicroscopy differ from bright-field microscopy?

A: Microscopes are used to observe objects at cellular level such as the nucleus, mitochondria and…

Q: What is the function of staining in light microscopy?

A: The light microscopy or light microscope represents a laboratory instrument required for the…

Q: What are the two parts used to carry the microscope?  What does Total Magnification mean?

A: Microscope is used in laboratory settings to obtain a clear and precise view of the specimens under…

Q: How do stains used for light microscopy compare withthose used for electron microscopy?

A: The micro-organism is the organism that is too small to be seen with the naked eye. It is viewed by…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.