You are trying to come up with a drug to inhibit the activity of an enzyme  thought to have a role in liver disease. In the laboratory the enzyme was shown to have a Km of 1.0 x 10-6 M and Vmax of 0.1 micromoles/min.mg measured at room temperature. You developed a competitive inhibitor. In the presence of 5.0 x 10-5 M inhibitor, the apparent Km of the enzyme was found to be 1.5 x 10-5 M. What is the Ki of the inhibitor?

The Michaelis-Menten equation is often used to describe the kinetic characteristics of an enzyme-catalyzed reaction. Vmax [S] U = Km + [S] where v is the velocity, or rate, Vmax is the maximum velocity, Km is the Michaelis-Menten constant, and [S] is the substrate concentration. A graph of the Michaelis-Menten equation is a plot of a reaction's initial velocity (vo) at different substrate concentrations ([S]). First, move the line labeled Vmax to a position that represents the maximum velocity of the enzyme. 300 V Next, move the line labeled 1/2 Vmax to its correct position. max 275 Then, move the line labeled Km to its correct position. 250 225 200 175 Michaelis–Menten curve 150 K a 125 m 100 1/2 V 75 max 50 25 10 20 30 40 50 60 70 80 90 100 [S] (µM) Incorrect Estimate the values for Vmax and Km. Vmax 175 µM/min Km 14 µM v (µM/min)

Acetylcholinesterase is an enzyme that catalyses the hydrolysis of acetylcholine to choline. After several purification methods,  44.0 µg of acetylcholinesterase was purified from a crude extract and was found to catalyse the conversion of 16.0 μmol of acetylcholine (the substrate) to choline (the product) in 9 mins. What is the specific activity of acetylcholinesterase in units μmol min-1 mg-1.   (Round your answer to the nearest whole number. (i.e. If you calculated 40.03, input your answer as 40. Input only the numerical value. Do not input the unit).

Hydrogen purification was conducted at 550 °C using a 3-mm-thick palladium sheet having an area of 0.25 m?. (a) Calculate the diffusion coefficient of hydrogen in palladium at this temperature. The preexponent and the activation energy are 12,000 J/mol and 6.5 x 108 m?/s, respectively. R = 8.3145 J/mol-K. (b) When the steady-state condition was attained, hydrogen concentrations at the high- and low-pressures side of the plate were 1.7 and 0.5 kg/m³. Compute the amount of hydrogen that passes through the sheet per hour (in kg/h).

An exposure assessment study was conducted in a nail salon. Previous studies have indicated that airborne concentrations of methyl methacrylate are approximately 1 ppm. If the LOD of the analytical method is 0.25 ug and sampling should be conducted at a flowrate of 0.01 LPM, what is the minimum amount of time the sample should be collected to achieve a detectable concentration? Note: Methyl Methacrylate MW=100.1 g/mol A. 11.2 Minutes B. 2.7 Minutes C. 8.5 Minutes D. 6.1 Minutes

In a study of the formation of HI from its elements,H₂(g)+I₂(g)→ 2HI(g) equal amounts of H₂ and I₂ were placed in a container, whichwas then sealed and heated.(a) On one set of axes, sketch concentration vs. time curves for H₂ and HI, and explain how Qchanges as a function of time.(b) Is the value of Qdifferent if [I₂] is plotted instead of [H₂]?

Although there are accurate graphical methods available for determining Vmax and Km of an enzyme-catalyzed reaction, sometimes these parameters can be quickly estimated by examining the values of Vo as [S] increases. Estimate the values of Vmax and Km of the reaction catalyzed by an enzyme in which the following data were obtained:

20B.2(b) The rate constant for the first-order decomposition of a compound A in the reaction 2 A→P is krr=3.56×10−7 s−1 at 25 °C. What is the half-life of A? What will be the pressure, initially 33.0 kPa after (i) 50 s, (ii) 20 min after initiation of the reaction?

1.2-MIB (Methylisoborneol) is one of the by-products of algae and is a representative trace pollutant that causes taste and smell of water. The isothermal adsorption equation of 2-MIB applied with activated carbon in water without organic matter was experimentally determined as follows.q = 1.48C^(0.65) Where q is ng 2-MIB / g activated carbon and C is the concentration of g / L 2-MIB (g / L).(n is 10 ^ -9) A. Calculate the amount of activated carbon (mg / L) required to reduce the concentration of 2-MIB to 5 g / L. It is assumed that the concentration of 2-MIB in water without organic matter is 50 g / L.B. The activated carbon adsorption column will be designed with a three-year operating period. If the density of activated carbon is 540 kg / m3 and the design flow rate per column effective cross-section is 10 m / hr, find the depth of activated carbon in the empty bed contact time (EBCT) and column. Here, it is assumed that there is no competitive adsorption that can interfere with…

In a study of the gas phase decomposition of nitrogen dioxide at 383 °C NO₂(g) →→→NO(g) + ½ O₂(g) the concentration of NO₂ was followed as a function of time. It was found that a graph of 1/[NO₂] versus time in seconds gave a straight line with a slope of 0.815 M-¹ s¹ and a y-intercept of 11.5 M-¹. Based on this plot, the reaction is [ ✓order in NO₂ and the rate constant for the reaction is M-¹ S-¹.