Acetylcholinesterase is an enzyme that catalyses the hydrolysis of acetylcholine to choline. After several purification methods, 44.0 µg of acetylcholinesterase was purified from a crude extract and was found to catalyse the conversion of 16.0 μmol of acetylcholine (the substrate) to choline (the product) in 9 mins. What is the specific activity of acetylcholinesterase in units μmol min-1 mg-1.
Catalysis and Enzymatic Reactions
Catalysis is the kind of chemical reaction in which the rate (speed) of a reaction is enhanced by the catalyst which is not consumed during the process of reaction and afterward it is removed when the catalyst is not used to make up the impurity in the product. The enzymatic reaction is the reaction that is catalyzed via enzymes.
Lock And Key Model
The lock-and-key model is used to describe the catalytic enzyme activity, based on the interaction between enzyme and substrate. This model considers the lock as an enzyme and the key as a substrate to explain this model. The concept of how a unique distinct key only can have the access to open a particular lock resembles how the specific substrate can only fit into the particular active site of the enzyme. This is significant in understanding the intermolecular interaction between proteins and plays a vital role in drug interaction.
Acetylcholinesterase is an enzyme that catalyses the hydrolysis of acetylcholine to choline. After several purification methods, 44.0 µg of acetylcholinesterase was purified from a crude extract and was found to catalyse the conversion of 16.0 μmol of acetylcholine (the substrate) to choline (the product) in 9 mins. What is the specific activity of acetylcholinesterase in units μmol min-1 mg-1.
(Round your answer to the nearest whole number. (i.e. If you calculated 40.03, input your answer as 40. Input only the numerical value. Do not input the unit).
Provided,
amount of acetylcholinesterase purified from the crude extract is 44.0 μg.
The amount of substrate (acetylcholine) converted by the enzyme is 16.0 μmol.
The time it took for this conversion to occur was 9 minutes.
For specific activity of acetylcholinesterase in units of we can use the following formula
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