Acetylcholinesterase is an enzyme that catalyses the hydrolysis of acetylcholine to choline. After several purification methods,  44.0 µg of acetylcholinesterase was purified from a crude extract and was found to catalyse the conversion of 16.0 μmol of acetylcholine (the substrate) to choline (the product) in 9 mins. What is the specific activity of acetylcholinesterase in units μmol min-1 mg-1.

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Acetylcholinesterase is an enzyme that catalyses the hydrolysis of acetylcholine to choline. After several purification methods,  44.0 µg of acetylcholinesterase was purified from a crude extract and was found to catalyse the conversion of 16.0 μmol of acetylcholine (the substrate) to choline (the product) in 9 mins. What is the specific activity of acetylcholinesterase in units μmol min-1 mg-1.  

(Round your answer to the nearest whole number. (i.e. If you calculated 40.03, input your answer as 40. Input only the numerical value. Do not input the unit).

Expert Solution
Step 1: Given data

Provided, 

  • amount of acetylcholinesterase purified from the crude extract is 44.0 μg.

  • The amount of substrate (acetylcholine) converted by the enzyme is 16.0 μmol.

  • The time it took for this conversion to occur was 9 minutes.

        For specific activity of acetylcholinesterase in units of bold italic mu bold italic m bold italic o bold italic l bold italic divided by bold italic m bold italic i bold italic n bold italic divided by bold italic m bold italic g bold italic comma we can use the following formula  bold italic S bold italic p bold italic e bold italic c bold italic i bold italic f bold italic i bold italic c bold italic space bold italic A bold italic c bold italic t bold italic i bold italic v bold italic i bold italic t bold italic y bold italic space bold italic left parenthesis bold italic mu bold italic m bold italic o bold italic l bold italic. bold italic m bold italic i bold italic n to the power of bold italic minus bold italic 1 end exponent bold italic. bold italic m bold italic g to the power of bold italic minus bold italic 1 end exponent bold italic right parenthesis bold italic space bold italic equals bold italic space fraction numerator bold italic left parenthesis bold italic A bold italic m bold italic o bold italic u bold italic n bold italic t bold italic space bold italic o bold italic f bold italic space bold italic P bold italic r bold italic o bold italic d bold italic u bold italic c bold italic t bold italic space bold italic f bold italic o bold italic r bold italic m bold italic e bold italic d bold italic space bold italic i bold italic n bold italic space bold italic mu bold italic m bold italic o bold italic l bold italic right parenthesis over denominator bold italic space bold italic left parenthesis bold italic T bold italic i bold italic m bold italic e bold italic space bold italic i bold italic n bold italic space bold italic m bold italic i bold italic n bold italic u bold italic t bold italic e bold italic s bold italic space bold italic cross times bold italic A bold italic m bold italic o bold italic u bold italic n bold italic t bold italic space bold italic o bold italic f bold italic space bold italic E bold italic n bold italic z bold italic y bold italic m bold italic e bold italic space bold italic i bold italic n bold italic space bold italic m bold italic g bold italic right parenthesis end fraction bold italic rightwards arrow bold italic left parenthesis bold italic 1 bold italic right parenthesis


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