Chemistry
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
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Acetylcholinesterase is an enzyme that catalyses the hydrolysis of acetylcholine to choline. After several purification methods,  44.0 µg of acetylcholinesterase was purified from a crude extract and was found to catalyse the conversion of 16.0 μmol of acetylcholine (the substrate) to choline (the product) in 9 mins. What is the specific activity of acetylcholinesterase in units μmol min-1 mg-1.  

(Round your answer to the nearest whole number. (i.e. If you calculated 40.03, input your answer as 40. Input only the numerical value. Do not input the unit).

Expert Solution
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Step 1: Given data

Provided, 

  • amount of acetylcholinesterase purified from the crude extract is 44.0 μg.

  • The amount of substrate (acetylcholine) converted by the enzyme is 16.0 μmol.

  • The time it took for this conversion to occur was 9 minutes.

        For specific activity of acetylcholinesterase in units of bold italic mu bold italic m bold italic o bold italic l bold italic divided by bold italic m bold italic i bold italic n bold italic divided by bold italic m bold italic g bold italic comma we can use the following formula  bold italic S bold italic p bold italic e bold italic c bold italic i bold italic f bold italic i bold italic c bold italic space bold italic A bold italic c bold italic t bold italic i bold italic v bold italic i bold italic t bold italic y bold italic space bold italic left parenthesis bold italic mu bold italic m bold italic o bold italic l bold italic. bold italic m bold italic i bold italic n to the power of bold italic minus bold italic 1 end exponent bold italic. bold italic m bold italic g to the power of bold italic minus bold italic 1 end exponent bold italic right parenthesis bold italic space bold italic equals bold italic space fraction numerator bold italic left parenthesis bold italic A bold italic m bold italic o bold italic u bold italic n bold italic t bold italic space bold italic o bold italic f bold italic space bold italic P bold italic r bold italic o bold italic d bold italic u bold italic c bold italic t bold italic space bold italic f bold italic o bold italic r bold italic m bold italic e bold italic d bold italic space bold italic i bold italic n bold italic space bold italic mu bold italic m bold italic o bold italic l bold italic right parenthesis over denominator bold italic space bold italic left parenthesis bold italic T bold italic i bold italic m bold italic e bold italic space bold italic i bold italic n bold italic space bold italic m bold italic i bold italic n bold italic u bold italic t bold italic e bold italic s bold italic space bold italic cross times bold italic A bold italic m bold italic o bold italic u bold italic n bold italic t bold italic space bold italic o bold italic f bold italic space bold italic E bold italic n bold italic z bold italic y bold italic m bold italic e bold italic space bold italic i bold italic n bold italic space bold italic m bold italic g bold italic right parenthesis end fraction bold italic rightwards arrow bold italic left parenthesis bold italic 1 bold italic right parenthesis


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