You are studying a transgenic mouse strain that expresses a GFP reporter gene under the control of cisacting regulatory elements that normally control agene needed for the early development of mice.Previous evidence from transcriptome sequencing(RNA-seq) indicates that mRNA for the gene of interest can be identified between days 8.5 and 10.5 of gestation. In your strain, GFP fluorescence can be seenfrom about day 8.75 until at least day 12.a. Explain the discrepancy between mRNA and protein expression.b. Would you expect GFP protein expression to indicate more accurately the normal onset of activityfor this gene or the normal cessation of this gene’sactivity? Explain.
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
You are studying a transgenic mouse strain that expresses a GFP reporter gene under the control of cisacting regulatory elements that normally control a
gene needed for the early development of mice.
Previous evidence from transcriptome sequencing
(RNA-seq) indicates that mRNA for the gene of interest can be identified between days 8.5 and 10.5 of gestation. In your strain, GFP fluorescence can be seen
from about day 8.75 until at least day 12.
a. Explain the discrepancy between mRNA and protein expression.
b. Would you expect GFP protein expression to indicate more accurately the normal onset of activity
for this gene or the normal cessation of this gene’s
activity? Explain.
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