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You are given a cell stock A. You make a 1:100 dilution and load 10µL diluted sample to a hemocytometer. After counting all 5 "one millimeter squares" you get an average of 60 cells. Then you decide to do a 1:1000 dilution to verify your counting. How many cells do you expect to get in average with 1:1000 dilution? Select one: a. b. 12 O c. 30 d. 20 To do a 1:100 serial dilution. This is what you will do: Select one: You take 1uL of original cell stock and add into 99 uL of 1XPBS to make a 1:100 dilution a. b. You take 90uL of cell stock and add that into a tube containing 10uL of 1x PBS. C. You take 99uL of cell stock and add that into a tube containing 10uL of 1XPBS. d. You take 10uL of original cell stock and add into 90 uL of 1x PBS to make a 1:10 dilution. You thoroughly mix the 1:10 dilution tube. Then you take 10uL out from 1:10 tube and add it into a new tube containing 90uL of 1×PBS.

You are given a cell stock A. You make a 1:100 dilution and load 10µL diluted sample to a hemocytometer. After counting all 5 "one millimeter squares" you get an average of 60 cells. Then you decide to do a 1:1000 dilution to verify your counting. How many cells do you expect to get in average with 1:1000 dilution? Select one: a. b. 12 O c. 30 d. 20 To do a 1:100 serial dilution. This is what you will do: Select one: You take 1uL of original cell stock and add into 99 uL of 1XPBS to make a 1:100 dilution a. b. You take 90uL of cell stock and add that into a tube containing 10uL of 1x PBS. C. You take 99uL of cell stock and add that into a tube containing 10uL of 1XPBS. d. You take 10uL of original cell stock and add into 90 uL of 1x PBS to make a 1:10 dilution. You thoroughly mix the 1:10 dilution tube. Then you take 10uL out from 1:10 tube and add it into a new tube containing 90uL of 1×PBS.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You are given a cell stock A. You make a 1:100 dilution and load 10µL diluted sample to a hemocytometer. After counting all 5 "one millimeter squares" you get an average of 60 cells. Then you decide to do a 1:1000 dilution to verify your counting. How many cells do you expect to get in average with 1:1000 dilution? Select one: a. 6 b. 12 с. 30 O d. 20 To do a 1:100 serial dilution. This is what you will do: Select one: a. You take 1uL of original cell stock and add into 99 uL of 1×PBS to make a 1:100 dilution b. You take 90uL of cell stock and add that into a tube containing 10uL of 1x PBS. c. You take 99uL of cell stock and add that into a tube containing 10uL of 1×PBS. d. You take 10uL of original cell stock and add into 90 uL of 1x PBS to make a 1:10 dilution. You thoroughly mix the 1:10 dilution tube. Then you take 10uL out from 1:10 tube and add it into a new tube containing 90uL of 1×PBS.
Transcribed Image Text:You are given a cell stock A. You make a 1:100 dilution and load 10µL diluted sample to a hemocytometer. After counting all 5 "one millimeter squares" you get an average of 60 cells. Then you decide to do a 1:1000 dilution to verify your counting. How many cells do you expect to get in average with 1:1000 dilution? Select one: a. 6 b. 12 с. 30 O d. 20 To do a 1:100 serial dilution. This is what you will do: Select one: a. You take 1uL of original cell stock and add into 99 uL of 1×PBS to make a 1:100 dilution b. You take 90uL of cell stock and add that into a tube containing 10uL of 1x PBS. c. You take 99uL of cell stock and add that into a tube containing 10uL of 1×PBS. d. You take 10uL of original cell stock and add into 90 uL of 1x PBS to make a 1:10 dilution. You thoroughly mix the 1:10 dilution tube. Then you take 10uL out from 1:10 tube and add it into a new tube containing 90uL of 1×PBS.
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