1. Determine the amount of dehydrated medium needed to prepare 50 nutrient agar plates. Include amount for 2 additional plates as excess to compensate for compounding losses.2. Directions: Suspend 28.0 grams in the 1000 mL distilled water. Heat to boiling to dissolve themedium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to45-50°C. If desired the medium can be enriched with 5-10% blood or other biological fluids.Mix well and pour into sterile Petri dishes.

Nutrient Agar is a general purpose, nutrient medium used for the cultivation of microbes supporting…

Answered: Determine the amount of dehydrated…

Determine the amount of dehydrated medium needed to prepare 50 nutrient agar plates. Include amount for 2 additional plates as excess to compensate for compounding losses.

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter1: The Clinical Laboratory

Section1.8: Laboratory Math And Reagent Preparation

Problem 13RQ

See similar textbooks

Related questions

Q: What alterations in gastrointestinal function occur in association with very young or very old age?

A: In addition to the mouth and anus, the gastrointestinal system includes the throat, esophagus,…

Q: How are congenital abnormalities of the urinary collecting system detected and treated?

A: The word congenital means any trait or disease present from birth. Hence the diseases detected at…

Q: hat would the results of an iron study be in a paitent with iron deficiency anemia?

A: Iron Deficiency Anemia Iron…

Q: How are laboratory and diagnostic tests used to evaluate renal function and disease?

A: The kidney plays a role in a number of processes, including the regulation of different body fluid…

Q: come up with two illustrations of objects that exhibit patterns in nature and write a brief…

A: Introduction The word pattern refers to any consistent design or arrangement. The pattern in nature…

Q: Galactosemia is an autosomal recessive human disease that is treatable by restricting lactose and…

A: Let, G - allele for normal galactosemia gene g - allele for recessive galactosemia allele. Since,…

Q: When measuring the volume of a liquid in a graduated cylinder, at which point should the measurement…

A: A graduated cylinder is used to meausre the volume of liquids. Liquids that are to be measured can…

Q: What characteristics cytochemical is positive in Hairy Cell Leukemia?

A: Hairy cell leukemia is a type of cancer in which the bone marrow makes too many lymphocytes (a type…

Q: What is mitrochondria?

A: The living, self-reproducing, structural and functional unit of the living organisms is called cell.…

Q: Low Power Magnification Short Description of Figure 2: Conclusion: Medium Power Magnification High…

A: Introduction Plants multicellular eukaryotic organisms. They have cell wall. Animals are…

Q: Provide a scientific example of evolution and/or natural selection. You can use human and/or animal…

A: Let us understand the process of evolution and the changes during evolutionary phases by an example…

Q: What are the essential characteristics of science?

A: Introduction: The physical world and its phenomena are included in the systematised body of…

Q: 1. Draw and label the Important parts of a hypothetical cell. 2. Enumerate the 2 major classes of…

A: Introduction An organelle is a subcellular structure that, like an organ in the body, has one or…

Q: 1. To produce a specialized-transducing mycobacteriophage construct, a cosmid bearing the allelic…

A: Cosmids :- These are the vectors, which are constructed by using hybrids of lambda…

Q: The pedigree shown is A) Autosomal Dominant B) Autosomal Recessive C) Sex linked D) Sex linked…

A: In the given pedigree, the trait skips generation i.e., two unaffected parents have affected…

Q: What is main objective for Waste Vegetable Treatment to produce electricity

A: Vegetable waste It is a biodegradable substance that is produced in large quantities. The waste…

Q: Knowing the pathogenesis of TB, what virulence factors would you expect to see in this microorganism…

A: Tuberculosis is a bacterial infection that affects the lungs. However, it can also attack other…

Q: Describe the chemistry of each step

A: Note: According to guidelines only one question is to be answered at once. Please repost the other…

Q: You are given a protein solution with a concentration of 0.15 mg/ml. We need 10 μg for an…

A: The high concentration solutions used for dilution are called stock solutions. A stock many contain…

Q: Antibody Panel: MNS e f M N S + + + + 0 0 0 + 0 + + + + + 0 + + + + + 0 + + + + + + + + + 0 8 00 0 +…

A: Step 1: Antibody screening for red blood cells (RBC) is done to test the presence of RBC antibodies…

Q: In which situation would passive transport not use a transport protein for entry into a a. water…

A: The solutes migrate down the concentration gradient from high concentration to low concentration in…

Q: What hormonal changes lead to menopause?

A: Menopause means the end of the menstrual cycle. Menopause generally occurs at the of 45 to 55. It…

Q: Bacillus anthracis has a length of 4.1 micrometers. Convert this value to scientific notation.

A: Scientific notation means way of expressing number from too large to small and opposite, mainly in…

Q: Demonstrate your understanding of industrial microbiology by explaining the involvement of in the…

A: Industrial microbiology is a well known field of microbiology in which various microorganisms are…

Q: 11. Design your own experiment: Does the number of spines on Holly leaves differ between male and…

A: Introduction Ilex, also known as holly, is the only genus still existing in the family…

Q: lon K+ K+ K+ K+ Na+ Na+ Na+ Ca++ Ca++ CI™ CI™ CI™ Concentration (mm) Intracellular 400 140 140 140…

A: The Nernst potential is the voltage that would balance the ion's uneven concentration across the…

Q: Select a specific type of birth control currently on the market (available). Identify how that birth…

A: Introduction : Birth control refers to the use of specific tools, medications, or surgical…

Q: What are the common causes of these general manifestations of gastrointestinal disorders: pain,…

A: The gastrointestinal system is a complex network of organs and tissues that work together to digest…

Q: List the ions that contribute to electrical signaling in neurons and what side of the neuron their…

A: Nerve cells also called neuron communicate by generating electric signal ans also through the…

Q: Can you show me how to do it? Show me the cross and explanation please. Testing for independent…

A: Introduction Genes can be linked or autonomous. When they segregate during formation, those genes…

Q: 3. What distinct organelles perform energy transformations? mog

A: Organelles are unique and well-organized structures found in living cells. Vacuoles, nuclei,…

Q: cell

A: In humans, the largest cell is ovum ,the human egg The smallest human cell is sperm cell. The…

Q: The Celsius scale is primarily used in climate studies and industry. True or False True False

A: Celsius is a measure of temperature and the most commonly used unit in the entire world. In…

Q: The Burmese Python, an invasive species, has been introduced to the Florida Everglades, largely…

A: Introduction An invasive species is a species that is not native to a particular area. An invasive…

Q: What are the risk factors and clinical manifestations for bladder cancer?

A: Bladder cancer is a type of cancer in which cells of the bladder called urothelial cells divides…

Q: True or false. The cell walls of plants prevent the process of cytokinesis.

A: Cell division is an essential process of the lifecycle of the cell in which the parent cells are…

Q: How can benign and malignant breast lumps be clinically differentiated?

A: Tumors that are benign form in one spot and do not spread to surrounding tissues. Tumors that are…

Q: constructing

A: NORMALITY ASSUMPTION : The normality is a property of random variable that is distributed according…

Q: In the metric system, the base unit of a liquid is the Multiple Choice о O kilogram. meter. All of…

A: In the metric system, the base unit of liquid is liter.

Q: Figure 1.8 Which of the following statements is false?A. Tissues exist within organs which exist…

A: Living things have a strong sense of order and organisation that may be studied on a scale from…

Q: Organelles Animal/Plant/Both Nickname (give a nickname that is related to it's function) Explanation…

A: According to Bartleby guidelines, we are supposed to answer first three subparts in case of multiple…

Q: Discuss how the biogeochemical cycle and the cycle of nutrient elements essential to life's…

A: Biogeochemical cycles mainly refer to the movement of nutrients and other elements between biotic…

Q: Largest unit of weight When comparing the milligram, gram, microgram, and kilogram, the largest unit…

A: Introduction In biology, you will often find a need to describe measurements of temperature or…

Q: O O C

A: Inheritance or heredity is passing on one trait from the parents to the progeny by either asexual or…

Q: Give the stratigraphic arrangement of rock layers as shown below, the circles represent places where…

A: The correct answer is option: B The therapsids are believed to be present between the Carboniferous…

Q: Hemoglobin is an enzyme containing amino acids linked by peptide bonds. Select one: True False

A: Introduction : Enzymes are biological macromolecular catalysts. Chemical reactions are accelerated…

Q: Q3.6. The farmer's experiment was widely considered to be well-designed and well-implemented. After…

A: Given information The harvest of blackberries is lower than usual. The farmer suspects that either…

Q: The monomers that make up proteins are called_________.a. nucleotidesb. disaccharidesc. amino…

A: Proteins are the polypeptide chain, which folds into a three dimensional (3D) structure to…

Q: How does population density affect the physiology and behavior of organisms?

A: In nature, there are various constraints on population number and expansion. Some are depending on…

Q: reason why open system has a limitation of collecting only 10 mL of blood?

A: Open and closed collection are two methods of collecting blood samples for lab testing. 1. Closed…

Concept explainers

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.

Question

1. Determine the amount of dehydrated medium needed to prepare 50 nutrient agar plates. Include amount for 2 additional plates as excess to compensate for compounding losses.

Directions: Suspend 28.0 grams in the 1000 mL distilled water. Heat to boiling to dissolve the
medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to
45-50°C. If desired the medium can be enriched with 5-10% blood or other biological fluids.
Mix well and pour into sterile Petri dishes.

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Follow-up Questions

Read through expert solutions to related follow-up questions below.

Follow-up Question

With the same directions:

Solution

by Bartleby Expert

SEE SOLUTION

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

Suspend 28.0 grams in the 1000 mL distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (1210C) for 15 minutes. Cool to 45-500C. If desired the medium can be enriched with 5-10% blood or other biological fluids. Mix well and pour into sterile Petri dishes. 1. Determine the amount of dehydrated medium needed to prepare 50 nutrient agar plates. Include amount for 2 additional plates as excess to compensate for compounding losses.
All recipes are given per litre unless specified. Lambda Broth (LB) Plates Top Agar 10 g tryptone 10 g Tryptone 2.5 g NaCl 2.5 g NaCl 15 g Agar 7 g Agar Make 5 mL aliquots, then autoclave. Lambda Broth (LB) Media Suspension Media 10 g tryptone 5.0 g NaCl 2.5 g NaCl 2 g MgSO4· 7H2O 50 ml 1 M Tris pH 7.5 In LB medium…
Given the following media recipe, pick the terms that apply (pick as many as are applicable). 10.0 g Meat extract 10.0 g Peptone 5.0 g Sodium chloride 15.0 g 1.0 L Agar Water Once media is cool, add 6% sterile sheep's blood Defined Complex Minimal Selective Differential
What is the minimal concentration (MIC) for disinfectant number 1? Just write the corresponding number including the decimal as written. ug/mL. Disinfectant 1 2 3 4 concentration 0.1 ug/mL +++ ++++ ++ +++ 0.2 ug/mL ++ ++ + ++ 0.5 ug/mL + + 1.0 ug/mL 5.0 ug/mL subculture 1 2 3 4 0.1 ug/mL ++++ ++++ ++++ ++++ 0.2 ug/mL ++++ ++++ ++++ ++++ 0.5 ug/mL ++++ ++++ ++++ 1.0 ug/mL ++++ 5.0 ug/mL Submit response
All recipes are given per litre unless specified. Lambda Broth (LB) Plates Top Agar 10 g tryptone 10 g Tryptone 2.5 g NaCl 2.5 g NaCl 15 g Agar 7 g Agar Make 5 mL aliquots, then autoclave. Lambda Broth (LB) Media Suspension Media 10 g tryptone 5.0 g NaCl 2.5 g NaCl 2 g MgSO4· 7H2O 50 ml 1 M Tris pH 7.5 In LB medium…
Prepare 50 plates of Sheep's Blood Agar using 100mm petri dish. Add 10% of blood to the media. (Manufacturer's instructions: Suspend 38 g of blood agar base dehydrated powder in 1 liter of distilled or deionized water.) Pour 25 mL of prepared medium per plate. Express answer to the nearest whole number. How many grams of the dehydrated powder should be weighed? Answer: What is the volume of distilled water that should be used in the preparation? Answer: What is the volume of sheep's blood that should be added to the sterilized and cooled blood agar base? Answer:
The reason that water for injection should be held on 80OC or above after distillation is: Select one: Inhibit bacterial division Keeps it sterile Chemicals will dissolve easily Prevents regrowth of Gram negative bacteria
On a paper, draw a schematic diagram on the Extraction of Flavonoids using the procedures stated below. Procedure: Extraction of Flavonoids Weigh 10 g of the shade dried plant sample and chop into small pieces. Macerate with 200 mL 70% ethanol for 3 days at room temperature (25°C). Filter the extracts in vacuum using Whatman filter. Evaporate to dryness. Weigh the residue and compute the percentage yield.
Explain this example of the following water quality serial dilution. Note the dilution factors and how you would calculate #’s from the incubated plates. ……A serial dilution yielded TNTC in the 1:1 plate and the 1:10 plate, 50 cfu’s in the 1:100 plate and 3 cfu’s in the 1:1000 plate . What is the concentration (CFU)/ml of the raw sample? Is water acceptable for potable or Recreational use? Explain. When do you use filtration rather than serial dilution
Disinfectants and Sterilization Procedures Complete the table by putting (+) for growth of microorganisms or (–) absence of growth of microorganisms. Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes 15 minutes Sodium hypochlorite 5% Sodium hypochlorite 0.05% Alcohol 70% Alcohol 40% Iodophor (Betadine) 10% solution Mouthwash Cetylpyridinium Chloride - 0.075% Sodium Fluoride - 0.05%
Is it safe to use 100g of Lye (NaOH) as a disinfectant at homes? Justify your claim by computing the percent composition. Claim: Yes/No Solution:
Which of the following can most likely increase the yield of recrystallized benzoic acid? CHOICES: A fluted filter paper was used during cold filtration. The receiving flask from hot filtration does not contain a small amount of solvent. Activated charcoal was not added to the solution. The solution was filtered immediately after hot filtration.