Why should a young culture be used in gram staining rather than an old culture? b. Why is it necessary to decolorize only for a few seconds

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter7: Basic Clinical Microbiology
Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing
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Why should a young culture be used in gram staining rather than an old culture?
b. Why is it necessary to decolorize only for a few seconds

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