Acid-Fast stain A) E. Coli -Morphology shape -Arragement -Acid-fast or non-Acid-fast B) S. Epidermidis -Morphology shape -Arragement -Acid-fast or non-Acid-fast

Principle/ technique used in Flagella Stain Method/ Preparation/ Procedure of Flagella Stain

Select all that apply to a negative stain:    1. involves a washing step 2. cells may be distorted or shrunken  3. uses an acidic or negatively charged dye which stains the background 4. uses multiple dyes in the procedure 5. uses only 1 dye in the procedure 6. involves fixing 7. does not involve fixing 8. cells will not be distorted or shrunken 9. does not involve a washing step 10. can show cell morphology, size, and arrangement 11. uses a basic or positively charged dye which stains the bacterial cells

a) explain in your own words how to do a nitrate reduction test to identify unknown bacteria   b) explain in your own words how to perform a phenol red broth (prb) test to help identify unknown bacteria

Abigail did not dry the top of her agar plate and stored it top up. Now she wants to count colonies but there is a smear over the entire plate. She was working with E. coli. Why does she have a smear?