Why is it difficult to accurately estimate Km and Vmax values from a Michaelis-Menten plot? Why are the benefits of estimating Km and Vmax values from a Lineweaver-Burk plo
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- what are the complications or the low-reliability in determining the value of KM using graphical methods from a Michaelis-Menten PlotWrite Cif only statement A is correct, Hif only statement B is correct, Eif both statements are correct, Mif both statements are incorrect. A. For the Lineweaver-Burk plot, the slope of the line reflects the ratio between the maximum velocity and the Michaelis constant. B. Meanwhile, the y-intercept is the maximum velocity.With respect to the concerted model, what is the L value? What is the c value?
- From the graphs given, can you find the Km from the Michaelis-Menten curve and the Lineweaver-Burke plot.Briefly explain why the Tm measured in the experiment is different from the Tm predicted from Marmur and Doty equation. please provide the references.Please discuss the value of international normalized ratio (INR) as a test. Why do you think this is more reliable that the ordinary PT.
- Using the rule of 300, what is the approximate rate?In a Lineweaver-burk plot, the Y-intercept is -1/KM True or False[S] (mM) v0 (mM/sec) 6 150 17 250 29 260 40 360 50 350 60 400 72 403 82 425 92 430 103 442 115 455 125 450 Km and Vmax obtained from the Lineweaver-Burk plot and linear regression analysis for the data in Table 2 are 16.9 mM and 503 mM respectively. Compare these values to the values obtained Vmax = 525.3 µM/sec Km = 20.70 µM . Which method, nonlinear regression or linear regression, is more tolerant to an experimental error? Note that lower substrate concentrations, which often have the greater error, have a greater effect on the slope of the line in the Lineweaver-Burk plot.
- Use MS Excel to plot and determine the terms being asked: Please insert the excel file, thank you For each V (mmol/min), determine the value of [S] (mM) if Vmax = 5 mmol/min. Show your calculations. Using MS Excel, plot [S] (mM) versus V (mmol/min). Draw a line corresponding to the given Vmax parallel the x-axis and extend your plotted line to show its approach to Vmax. Provide your final plot [S] (mM) V (mmol/min) 10 1.2 1.7 2.1 2.2 2.5 Determine the values of Km and Vmax for the decarboxylation of β-keto acid given the following data. Please show and label your plot. Show your calculations and box your final [S] in mol/l V in mM/ min 2.500 0.588 1.000 0.500 0.714 0.417 0.526 0.370 0.250 0.256What are the predictions given by the Hardy–Weinberg law?How would you calculate the expected values from the observed in order to use the chi-squared test?