Km and Vmax obtained from the Lineweaver-Burk plot and linear regression analysis for the data in Table 2 are 16.9 mM and 503 mM respectively. Compare these values to the values obtained Vmax = 525.3 µM/sec Km = 20.70 µM . Which method, nonlinear regression or linear regression, is more tolerant to an experimental error? Note that lower substrate concentrations, which often have the greater error, have a greater effect on the slope of the line in the Lineweaver-Burk plot.
Km and Vmax obtained from the Lineweaver-Burk plot and linear regression analysis for the data in Table 2 are 16.9 mM and 503 mM respectively. Compare these values to the values obtained Vmax = 525.3 µM/sec Km = 20.70 µM . Which method, nonlinear regression or linear regression, is more tolerant to an experimental error? Note that lower substrate concentrations, which often have the greater error, have a greater effect on the slope of the line in the Lineweaver-Burk plot.
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter3: Biosignals And Noise
Section: Chapter Questions
Problem 5P
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Km and Vmax obtained from the Lineweaver-Burk plot and linear regression analysis for the data in Table 2 are 16.9 mM and 503 mM respectively. Compare these values to the values obtained Vmax = 525.3 µM/sec Km = 20.70 µM
. Which method, nonlinear regression or linear regression, is more tolerant to an experimental error?
Note that lower substrate concentrations, which often have the greater error, have a greater effect on the slope of the line in the Lineweaver-Burk plot.
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