Why does casein precipitates upon the addition of acetic acid? Why is milk used as an antidote in heavy metal poisoning? Can you identify which specific amino acid/functional group provides a positive result on the following tests? a. Biuret test b. Ninhydrin test c. Denaturation tests d. Xanthoproteic test
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Direction: Answer these quide questions thoroughly please and explain. Thank you so much.
Topic: Isolation and Characterization of Casein
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- A. Explain why it is essential to digest all wild-type pQE. 1.-CRYGD. B. How would you make 50 mL o 1% weight per volume (w/v) agarose solution?MATCHING TYPE; Match A to B A. Acrolein tesT Benedicts test Biuret test Iodine test Barfoed’s test Molisch test Seliwanoff’s test Fehling’s test Salkowski’s test Furter-Meyer test B. A. The test for the presence of cholesterol. B. The test for reducing sugars in acidic solution. C. The test is used to detect cholesterol in a solution D. The test for the presence of starch/amylose. E. The test for presence of reducing sugars in alkali solution. F. The general test for carbohydrates. G. This method is used to detect the presence of tocopherols H. The test to distinguish monosaccharides from disaccharides I. The test for the presence of fats or glycerin. J. The test to determine ketohexose from an aldohexose.A. Detecting Dextrin in Starch 1. Iodine Test for Starch Describe any change that took place when Iodine was added. 2. Preparation of Dextrin from Starch a. Describe the color of starch while being heated. b. Describe the change upon the addition of Iodine. What color indicates the presence of dextrin? c. Compare the result obtained with Procedure 1. d. Describe the color change when Iodine was added to the solution of dextrin from the supply shelf. B. Reduction test for Sugars 1. FEHLING'S TEST SUGAR 1. Glucose 2. Sucrose 3. Maltose 4. Fructose 5. Lactose 6. Galactose 2. BENEDICTS TEST SUGAR 1. Glucose 2. Sucrose 3. Maltose 4. Fructose 5. Lactose 6. Galactose 3. BARFOED'S TEST SUGAR 1. Glucose 2. Sucrose 3. Maltose 4. Fructose 5. Lactose RESULT RESULT RESULT
- 1. What is this lipid structure, give its role/ function, and enumerate its hydrolysis products or components. 2. Explain which hydrolyzed component will be positive in the Acrolein test? Ninhydrin test?1. The principle behind the test for phosphate is oxidative reaction, conversion of organic form to inorganic phosphate and a precipitation reaction. A. True B. False 2. Sakaguchi test determines amino acids with indole groups present. A. True B. False19. The pH indicator in the carbohydrate metabolism test was a. methyl red b. eosin methylene blue c. neutral red d. phenol red e. bromthymol blue 20. Which of the following enzymes would be indicated by the sheep blood agar plate? a. catalase b. gelatinase c. citrate permase d. hemolysins e. lipase 21. The pH indicator in your EMB agar was plate. a. phenol red, selective b. methyl red, enrichment c. bromthymol blue, differential d. neutral red, selective e. eosin methylene blue, differential and it is an example of a inscloudgate.net/files/71e091b2-95...riXAGSX7kihG5XaOhFABsP7PBUexzlaVKNAmC
- &. How many milldiners of a 2wv Lidocaine HCI stock selution in water should be used in preparing 30 ml of a 0.5% wiv Lidocaine HCI solution? . From the following formula for antificial tears, caleulate the quantity of each ingrodient roquired to prepare half donen of the 0ml containers. Poyviny alcohol 149 Povidone Chiorobutanol Serile sodum chioride soution a.%. s to 100 m How many milldiners of water should be added to 60 ml. of a 2% wiv agqueous solution to prepare a 1% wiv solution?1. List down the amino acids in the following format Amino Acid Physiological Significance 3 letter Code 1 letter code Chemical Structure 2. List down the different qualitative and quantitative tests for Amino Acids Test Reagents Principle Positive Reaction/ResultB. COLOR REACTIONS. Give the ideal observation for the positive results of the following samples to complete the table below. Write NO RXN if the sample gives negative result for the given tests BIURET XANTHOPROTEIC NINHYDRIN SULFUR SAMPLES TEST TEST TEST TEST Formation of violet- Cysteine NO RXN colored product Formation of red- Tryptophan NO RXN colored solution Formation of Formation of violet- Keratin black colored product precipitate B - Alanine NO RXN NO RXN
- I. True or False ____________1] Mutarotation affects the reducing property ofcarbohydrates.____________2] Anthrone test can be use to test for the helicalconfiguration of a polysaccharide.____________3] Upon treatment with phenylhydrazine reagent & hydrolysis, maltose gives glucuronic acid and glucosazone.1. Provide the Principles and detailed procedures of the following tests for the "Color Reaction of Proteins". A. Biuret Test B. Xanthoproteic Test C. Millon's Test D. Ninhydrin Test E. Hopkins-Cole Test F. Sulfur Test G. Heller's Test2. What sample shows a positive result with the Molisch Test? Why did this sample give a positive result? 3. What is the chemical basis of the Xanthoproteic Test? 4. Egg white and milk are used as antidotes for heavy metal poisoning. Explain. 5. According to advertisements, commercial shampoo or conditioner contains “protein." Suggest a test that could be done on the product to check this claim? Explain your answer.