Why do E. coli cells with a defective lacZ gene fail to show galactoside permease activity after the addition of lactose in the absence of glucose?

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Q: List the two components of the active Telomerase holoenzyme.

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Q: Why don't E. coli cells with a faulty lacZ gene display galactoside permease activity when lactose…

A: Introduction :- Escherichia coli bacteria can be found in the environment, foods, and people's and…

Q: A mutated TBP protein Explain: (a) What is the process affected? (b) What is the Effect on the…

A: TATA-Binding protein or TBP attaches specifically to particular sequence of DNA called TATA box.…

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A: Antisense RNA (asRNA), also referred to as antisense transcript,natural antisense transcript or…

Q: It has been proposed that thiogalactoside transacetylase (LacA in the lactose operon) plays a role…

A: The lactose operon is an operon required for the transport and metabolism of lactose in E.coli and…

Q: What would be the expected effect of attaching an ER signal sequence to a cytosolic protein?

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Q: A Cys residue is required for the transport function of a bacterial lactose transporter that is…

A: N-Ethylmaleimide (NEM) has been much used for the chemical modification of proteins, and  it is…

Q: The lac genotypes are as shown below: P+OcZ-Y+A+// P¯O+Z+Y+A+ (i) The lac operon consists of three…

A: Introduction The lactose operon (also known as the lac operon) is a group of genes present in E.…

Q: In the: Inhibition of splicing by ribozymes Explain: (a) What is the process affected? (b) What is…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

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A:

Q: Which of the proteins: the CAP or the lac repressor, does the eukaryotic nuclear receptor for the…

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Q: What is catabolite repression? How does it allow a bacterial cell to use glucose in preference to…

A: Microorganisms with catabolite repression can easily adapt to a chosen (rapidly metabolizable)…

Q: What if the cell found itself in an environment that contained ampleamounts of both lactose and…

A: Ans: The lactose and glucose are used by cells as a source of energy.

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A: Ca2+ ATPase is a transmembrane protein found in the sarcoplasmic reticulum of the muscle cells. It…

Q: Clary Fray used the pET vector system to express her prokaryotic amylase enzyme. She added peptone…

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Q: Where in the cell would you expect to find lysosomal hydrolases if the enzyme that adds phosphate…

A: Our cell contains numerous proteins and each protein has a target location. The location of the…

Q: I have this strain of e coli.  Is P+ o+ Z+ Y+ / I- P+ oC Z- Y+ Will beta-galactosidase and…

A: Lac operon constitutes a set of structural genes regulated by a common promoter in bacteria.

Q: Clary Leonhart used the pET vector system to express her prokaryotic amylase enzyme. added peptone…

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Q: hat is the nature and likely location(s) of a mutant that would, 1)allow constitutive expression of…

A: The lac operon is an operon or group of genes with a single promoter (transcribed as a single mRNA).…

Q: Describe how one might determine which proteins in E.coli are repressed when a culture is shifted…

A: Introduction  Gene expression is the process through which information from a gene is used to make a…

Q: Mabelle used the pET vector system to express her prokaryotic amylase enzyme. She added IPTG into…

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Q: Researchers isolated a yeast phosphofructokinase (PFK) mutant in which a serine at the…

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Q: Researchers isolated a yeast phosphofructokinase (PFK) mutant in which a serine at the…

A: Introduction: Glycolysis is the sequence of ten enzyme-catalyzed reactions that converts glucose…

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Q: How is the activation of the GAL1 gene prevented in thepresence of galactose and glucose?

A: The process wherein the expression of GAL genes is inhibited in the presence of glucose is known as…

Q: Mutations in the Galactose-1-Phosphate Uridyl Transferase Gene Can you use RFLP to identify this…

A: Mutation --It is an alteration in the sequence of  nucleotides in a genome of an organism , virus…

Q: what state will the prokaryotic repressor protein take in the place of lactose

A: Lac operon is the segment of DNA which contains regulatory gene, structural gene, promoter and…

Q: Yeast cells are eukaryotes, and they require a functional secretory pathway to grow and to maintain…

A: The signal recognition particle(SRP) is a very highly conserved and expressed ribonucleoprotein…