Chemistry
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
Bartleby Related Questions Icon

Related questions

Question

The technician then plates 0.1mL of the last three dilutions in duplicate. Why are the last three dilutions plated, as opposed to only one dilution? Why are the dilutions plated in duplicate?

Dilution Scheme to Achieve 100 PFU/Plate
10 μιL
10 μι
66.66 µl
100 μL
100 μL
100 μι
1
1
1
1
1
1
100
100
15
10
10
10
Dilution #
1
3
4
6.
LB (Diluent) µl
990
990
933.34
900
900
900
Dilution Factor
102
104
1.5x105
1.5x106
1.5x107
1.5x108
PFU/0.1mL
1.5x107
1.5x105
104
103
102
10
1.5 x 1010
PFU/mL
expand button
Transcribed Image Text:Dilution Scheme to Achieve 100 PFU/Plate 10 μιL 10 μι 66.66 µl 100 μL 100 μL 100 μι 1 1 1 1 1 1 100 100 15 10 10 10 Dilution # 1 3 4 6. LB (Diluent) µl 990 990 933.34 900 900 900 Dilution Factor 102 104 1.5x105 1.5x106 1.5x107 1.5x108 PFU/0.1mL 1.5x107 1.5x105 104 103 102 10 1.5 x 1010 PFU/mL
Expert Solution
Check Mark
Knowledge Booster
Background pattern image
Chemistry
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Similar questions
SEE MORE QUESTIONS
Recommended textbooks for you
Text book image
Chemistry
Chemistry
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Cengage Learning
Text book image
Chemistry
Chemistry
ISBN:9781259911156
Author:Raymond Chang Dr., Jason Overby Professor
Publisher:McGraw-Hill Education
Text book image
Principles of Instrumental Analysis
Chemistry
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Cengage Learning
Text book image
Organic Chemistry
Chemistry
ISBN:9780078021558
Author:Janice Gorzynski Smith Dr.
Publisher:McGraw-Hill Education
Text book image
Chemistry: Principles and Reactions
Chemistry
ISBN:9781305079373
Author:William L. Masterton, Cecile N. Hurley
Publisher:Cengage Learning
Text book image
Elementary Principles of Chemical Processes, Bind...
Chemistry
ISBN:9781118431221
Author:Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:WILEY