While neither grows in this generic environment, you are able to grow Organism A in this media with some minor modifications to the culture settings. Based on this ability to grow Organism A, but not Organism B, what would be a way to characterize this media with respect to these organisms? O tis both selective and differential for Organism B O Itis selective for Organism A O tis selective for Organism B O tis differential for Organism B O tis differential for Organism A

1. Plate count data are used to calculate microbial population density as colony forming units (CFUs) per milliliter of culture. Culture density (CFU/mL) = number of colonies (dilution factor) x (volume plated, in mL) Use these data below to calculate the CFU/mL in this sample of bacteria after 1 hr of growth. **Important to know for these types of questions - only use colony counts that fall between 10 and 300. TNTC = too numerous to count, so many colonies that you can't distinguish them to count. Use the plates with numbers of colonies that can be counted, calculate the CFU on each one, and then calculate the average between all of them. Looking for more assistance? Read section 7.3 in the textbook. Time 1h Dilution (before plating) Volume spread on plate 0.1 mL 0.1 mL 0.1 mL 0.1 mL none 1:10 (1 x 10-¹) 1:100 (1 x 10-²) 1:1,000 (1 x 10-³) Number of colonies observed (replicate plates) TNTC 499 83 10 TNTC 518 87 8 TNTC indicates too numerous to count. *Cell density could not be…

All of the following are valid measurements of growth except: A. Counting viable cells B. Estimating the weight of cells C. Determining the dry weight of cells D. Streaking for single colonies E. Measuring some cellular component F. Counting the total number of cells

Geneticists Identify Mutant Bacteria bythe Presence or __________ of ColoniesUnder Specific Growth Conditions