Inoculation Positive Results Negative Purpose Results Test Medium Reagents Mannitol Salts Loop Agar (MSA) Plate Needle Gelatinase Test (Gelatin Hydrolysis) Blood Agar Plate (Hemolysis) Loop EMB Agar Plate Loop Streak MacConkey Agar Loop Streak Plate Loop Streak Oxidase Test

Explain the following tests using Pseudomonas aeruginosa.  Describe the purpose, reagents used and the results using Pseudomonas aeruginosa.

Can you help me?

How can I explain the results of my findings in lamen terms? 1 - Name of Test Type of Medium 2 - Color Before Incubation 3-Color After Incubation? 4 – Results Positive/ Negative? 5 - Reagents Added Lactose Fermentation test Phenol Red Lactose (PRL) Red Color: Yellow   Gas Bubble: Yes Positive Phenol Red to detect color change (added) Glucose Fermentation Test Phenol Red Glucose (PRG) Red Color: Yellow   Gas Bubble: Yes Positive Phenol Red to detect color change (added) Citrate Utilization Simmons Citrate Green Color: Blue Positive Bromthymol Blue (added) Indole Test Media = SIM Clear Indole: Color: Clear Negative For Indole, we add Kovac’s   Hydrogen Sulfide Test Media = SIM clear Hydrogen Sulfide: Color: Clear Negative Iron already added Methyl Red Test (MR test) Media = MRVP Broth Clear Color: Yellow Negative We add Methyl Red Reagent Voges-Proskauer Test (VP test) Media = MRVP Broth Clear…

Solve the identity of an unknown bacterial specimen by creating a dichotomous key.   Categories: Gram stain, Mannitol salt agar,MacConkey agar, Catalase, Oxidase, Casein hydrolysis, Nitrate reduction, Citrate test, Urease test, Sim Medium Streptoccocus ID: 1) Bile Esculin Test 2) Bacitracin Test  3) Blood Agar Plate NaCl Broth Staphylococcus ID: 1) Mannitol Salt Agar 2) DNA Hydrolysis 3) Coagulase Test 4) Novobiocin Test    Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes

In the image attached is a unknown bacteria lab. The gram stain result and growth result is already given to us and is shown. What other tests (1-2 examples) could we use to follow up this test. Explain why we should use those tests with justification.

Nutrient agar and Gram stain results for the unknown lab attached.

Out of all these tests, which 2 would help further understand this unknown lab. (Gram Stain and Growth Result is already done) Possible Tests (pick 2 that would help know the unknown bacteria) Gram stain morphology & arrangement color and growth characteristics on agar 37C, 30C and 25C (room temp) CAMP test starch hydrolysis test bacitracin susceptibility test glucose, lactose, sucrose, maltose fermentation tests methyl red/MR test Mannitol MacConkey Phenol Red Vogues-Proskauer/VP test citrate utilization test urea hydrolysis test phenylalanine deaminase test SIM test (Sulfide/Indole/Motility) Nitrate reduction test Oxidase test Catalase test