Bovine intestinal alkaline phosphatase is an enzyme that normally operates in the intestines of cattle and has the function of breaking down a variety of phosphorylated compounds found in their food. Alkaline phosphatase can hydrolyze the artificial substrate p-nitrophenylphosphate (p-NPP). PNPP, which is colorless, turns yellow when de-phosphorylated by alkaline phosphatase to form the product, p-nitrophenol (p-NP). This reaction is summarized as follows: alkaline p-NPP (colorless) + H20 p-NP (yellow) + inorganic phosphate phosphatase

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Nitrogen metabolism

Nitrogen metabolism is the synthesis, utilization or uptake, breakdown, and excretion of nitrogenous compounds in the body. The nitrogen metabolism encompasses nitrogen fixation, biosynthesis, and the breakdown of amino acids, purines, and pyrimidine. Since nitrogen-containing compounds are required for various life processes, nitrogen is an important element of life.