Which of the following reagents will you include in the blank (negative control) for this experiment? 00000 Alkaline phosphatase Buffer solution: 50mM Tris-HCl, pH=10 Vitamin C Acid Molybdate Distilled water Rhino horn
Q: Why is the enzyme added last? Why can’t it be added at the start and the rates measured sometime…
A: For an enzyme catalysed reaction, the graph of the initial rate of reaction vs substrate…
Q: Show the structure of a DNA where the lead strand is ATCG. Show H-, glycosidic, phosphoester…
A: DNA is Nucleic acid that consists of two strands that run in opposite directions. One of the stand…
Q: Which is the proper order of permeability the following solutes would show when it moves across a…
A: Cell membrane is found in both animal and plant cells. It is also called as plasma membrane which…
Q: The coenzyme pyridoxal phosphate (PLP) in its active form has all the following characteristics…
A: Pyridoxal phosphate (PLP) is the coenzyme form of Vitamin B6 (pyridoxine). PLP is involved in the…
Q: What serves as the basis for the differences between large carbohydrates, proteins, and nucleic…
A: All biological molecules are organic molecules, which means they all contain carbon atoms. Hydrogen,…
Q: A) Is this reaction ( in picture provided) in equilibrium? B) If it is not then ,what is ∆G' at…
A: Gibbs free energy change, equilibrium constant and reaction quotients are values for a reaction…
Q: Consider the following DNA strand with the following nucleotide sequence: 3’-ATATCAGAGAATATCA-5’…
A:
Q: The template for protein synthesis is: the DNA template strand an mRNA strand a protein primer O the…
A: Introduction: The term 'Genetic code' is coined by George Gamow which consists of a sequence of…
Q: Describe how the movement of electrons along the electron transport chain leads to the generation of…
A: Introduction: The electron transport chain present in the inner membrane of the mitochondria…
Q: Another question about tecniques that im getting confused over Can someone explain me which one…
A: Trypsin and chymotrypsin are protease enzymes that cleave the internal peptide bonds in a peptide.…
Q: Describe the composition of bacterial cell walls.
A: The cell wall in bacteria tends to form a stable frame of constant thickness all around cell and is…
Q: 4. Below is a polypeptide with an unknown number of amino acids. On the diagram below: a. Circle…
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…
Q: Briefly discuss the ethics of recombinant DNA technology.
A: DNA means deoxyribonucleic acid. Recombinant DNA technology involves the creation of artificial DNA…
Q: Which of the following are properties of membranes? Electrically polarized Hydrophobic exterior…
A: The cell membrane is principally composed of a lipid bilayer. The membrane is selectively-permeable…
Q: What biomolecules can be found within a living cell? Define each and talk about how they work in…
A: A living cell is the basic unit of life. It is capable of independent existence (as is the case of…
Q: Which of the following is incorrect about the ATP synthase? O a. The Fo and F₁ components are…
A: ATP synthase or FoF1ATPase is an ubiquitous enzyme that uses the transmembrane electrochemical…
Q: What distinguishes metalloproteins from glycoproteins in particular?
A: Some proteins contain one or more chemical groups(other than amino acids) covalently linked to them.…
Q: What are the functional characteristics and structural arrangements of the biomolecules found inside…
A: A cell is a basic unit of life. It is capable of independent existence(single cell microorganisms)…
Q: 1. Assume that the ribosome has just catalyzed the formation of a peptide bond, but the ribosome has…
A: Translation of the nucleotide bases sequence in the mRNA to the amino acids results in the formation…
Q: Chemistry 1. Explain why gluconeogenesis is NOT just a reversal of glycolysis.
A: Hi! Thanks for your question. As you have posted multiple questions and have not mentioned which one…
Q: Which is NOT a phospholipid? cerebroside phosphatidylinositol cardiolipin phosphatidylethanolamine…
A: Phospholipids are the compounds that are composed of two fattyacids, Glycerol or other alcohol group…
Q: Find an example of an enzyme. Answer the following questions about the enzyme that you chose. a)…
A: Enzymes are the protein molecules that speed up biochemical reactions and accelerate the metabolic…
Q: Why is oxytocin sometimes administered to a woman following parturition?
A: Oxytocin is a hormone synthesized in the hypothalamus, and carried to the posterior pituitary gland.…
Q: 2. Draw a graph to calculate the initial rate of reaction due to enzyme X in absorbance…
A: Glucose dehydrogenase (GDH) is oxidoreductases enzyme which catalyzes the conversion of glucose to…
Q: The genetic code is both universal and degenerate. Explain how these aspects are an advantage, but…
A: Proteins are made up of amino acids encoded by codons. Each codon consist of three nucleotide which…
Q: How can one confirm/investigate the type of inhibition exerted by azt on the hiv reverse…
A: 3'-azido-3'-deoxythymidine (AZT) is a Thymine analogue. It inhibits HIV reverse transcriptase by…
Q: If acyetal-CoA dehydrogenase was unavailable, malonyl-CoA could not be made from aceytal-CoA. That…
A: There is no enzyme called acyetal CoA dehydrogenase or acetyl CoA dehydrogenase. The enzyme that…
Q: 1. What is the possible identity of the amino acid? 2. What is the isoelectric point of AA? [Select]…
A: The amino acids are composed of a central carbon, an alpha-amino group, an alpha-carboxylic group,…
Q: Differentiate DNA and RNA.
A: Nucleic acids are biomolecules that store, express, and transfer genetic information. A nucleic acid…
Q: Without checking the accuracy of a given Pipetman, would you pre- dict that it is better to use a…
A: Micropipette are the tools used for measuring, transfering a very fine volume in the range of…
Q: Write down the values in bold print. You are isolating a protein with a pl of 8.5 using cation…
A: As given in the question, a protein with pI of 8.5 was isolated using cation exchange…
Q: Illustrate and compare the primary and secondary structural levels of nucleic acids.
A: Nucleic acids are polymers consisting of nucleotide-named monomers. A nucleotide consists of a…
Q: Materials: • Single colony of E. coli cells to be transformed • LB medium • 0.1 M CaCl2, ice cold…
A: The most common bacterial species for transformation is E.coli but their natural competency (copy…
Q: Below is a diagram of the canonical Wnt signalling pathway. Numbers 1-7 refer to different molecules…
A: The Wnt signaling pathway is known for its role in cell differentiation, transcriptional regulation…
Q: How much DNA would you have after 30 cycles if you started with one DNA strand? a. 1.074 x 109 b.…
A: Polymerase chain reaction or PCR is a technique to amplify any segment of DNA in a template…
Q: At the end of the transformation and cell recovery steps, you realized that you are out of glass…
A: Transformation is a process by which cells uptake foreign DNA from the outside of the cell. This is…
Q: so for this biology homework assignment I have to explain why I got this wrong and how to get right…
A: Polymer Macro molecules are the complex structures that are formed by joining together of monomer…
Q: in a randomly mating laboratory population of Drosophila, 4% of the flies have black bodies, and 96%…
A: Since there are multiple questions, I will answer the first question.
Q: Which of the following is incorrect about the conversion of pyruvate (with 3 C) and CO₂ to…
A: In gluconeogenesis, glucose is synthesized from non-carbohydrate sources e.g., amino acids,…
Q: 1. Classify the 20 common amino acids C. According to Nutritional Requirements g. Non-essential h.…
A: Amino acids are biomolecules that have an amino group and a carboxyl group attached to the same…
Q: 2. a. How do the majority of proteins that exist outside cells get outside cells? Explain the…
A: There are different proteins inside the living system that needs to be sent to different parts in a…
Q: UDP- glucose 12. pathway glycogen ATP+ HCO amino acid 9. metabolite glu 'NH. & metabolite ornithine…
A: The pathways shown in the image represents integration of metabolism which showcase that all the…
Q: u have a 0.75 mg/mL solution of bovine serum albumin (BSA). You add…
A: Bovine Serum Albumin or BSA is a standard protein used to plot standard curve for measuring the…
Q: Sort the following metabolic enzymes according to those involved only in glycolysis and those…
A: Glycolysis is a process in which glucose is oxidized & is converted to pyruvate and in that…
Q: How many amino acids are in the following peptide? H₂N H3C ZI CH3 IZ O=C CH3 `N H HH OH | LOH
A: A polypeptide chain constitutes of amino acids are linked together via peptide bonds. The proteins…
Q: Which amino acid has a guanidino group? Histidine Arginine Tryptophan Aspartic acid Which amino acid…
A: Introduction: Amino acids are organic molecules that contain a carboxyl and an amino group with a…
Q: Imagine that you are Gram staining a mixed culture of both gram-positive and gram-negative bacteria.…
A: The bacteria cell envelope is a complex multilayered structure and based on this structure the…
Q: Which of the following is incorrect about the peptide Arg-Asp-Cys-Tyr-Gln-Arg-Glu? O a. Its…
A: The peptide given is represented in single letter codes as: RDCYQRE Its structure is:
Q: Please explain the topic about bunsen burner in the photo attached
A: In several biochemical processes, including sterilization and combustion, the material or molecules…
Q: Draw the following: A. a phosphatidylcholine with two oleic acid as the fatty acids B. a…
A: Lipids are structurally diverse biomolecules that have two things in common: low solubility in water…
Step by step
Solved in 2 steps
- You have been provided with stock solutions of: stock A: 0.06 M sodium pyrophosphate buffer pH 8.5 stock B: 3 M ethanol stock C: 0.015 M NAD+ stock D: milli Q water Determine the volume you will need of each solution to prepare a buffer of with a final volume of 60 mL containing 10 mM sodium pyrophosphate pH 8.5, 100 mM ethanol, 1 mM NAD+. i.e. volume of stock A = _________mL volume of stock B = _________mL volume of stock C = _________mL volume of stock D = _________mL Show your calculations to arrive at your answers.Given the following information, calculate the total activity in the undiluted protein sample. Activity of 1 ml of diluted sample = 0.5 Total volume of sample = 5 ml Dilution factor = 10 25 50.5 250 2.5Using DEAE-cellulose as ion exchange resin, indicate the starting and ending pH for the narrowest experimental pH range used to separate an amino acid mixture consisting of Gln, Leu and Lys Starting pH: _____ Ending pH: _____
- Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) buffer at pH 7 with 400 mM NaCl. A A purified protein is in a Hepes dialysis membrane tube holds a 5.0 mL sample of the protein solution. The sample tube floats in a beaker containing 0.50 L of the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na*, CI", and Hepes) can to diffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis. Calculate the final concentration of NaCl in the protein sample once the dialysis has come to equilibrium. Calculate the final NaCl concentration in the 5.0 mL protein sample after dialysis in 300 mL of the same Hepes buffer, with 0 mM NaCl, twice in succession. [NaCl] after a single dialysis: [NaCl] after a double dialysis: mM mMN-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic A purified protein is in a Hepes acid) buffer at pH 7 with 375 mM NaCl. A dialysis membrane tube holds a 2.0 mL sample of the protein solution. The sample tube floats in a beaker containing 1.00 L of the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na+, Cl-, and Hepes) can to diffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis. Calculate the final concentration of NaCl in the protein sample once the dialysis has come to equilibrium. Calculate the final NaCl concentration in the 2.0 mL protein sample after dialysis in 150 mL of the same Hepes buffer, with 0 mM NaCl, twice in succession. [NaCl] after a single dialysis: [NaCl] after a double dialysis: mM mM
- 10 tab of acetaminophen 500 mg molar absorptivity= 14520 @ 243nm. The total weight of the 10tab is 5278 mg. I take a sample equivalent to 100mg of tablet and I diluted it in 100mL, I took an aliquot of 10mL and I diluted it in 250mL, of these I took 25mL and I diluted them in 250mL. Calculate the weight per tablet. Absorbance = 0.9443 mL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following values. The substrate molecular weight is 125 g/mol. Calculate the following values: Substrate Volume in mL Dilution Factor Substrate number of moles Substrate concentration for the diluted solution in mM Substrate concentration for the diluted solution in mole/L Substrate concentration for the diluted solution in mg/mLcan you show how to calculate the amount of two components for the required volume of a buffer with the receipes? Prepare enough 10X stock solution § Prepare 1LProportions§ 0.25M tris§ 1.92M glycine§ pH 8.3§ 1% (w/v) SDSInstructions to prepare 100mL of 10X running buffer1. Weigh 3.02g of Tris base and 14.42g of glycine into ~90mL of ddH2O in a clean beaker and stir todissolve.2. Check the pH with a calibrated pH metre once dissolved.§ The pH of the buffer should be 8.3. pH adjustment is not required if between 8.1-8.5.3. Add 5mL 20% (w/v) SDS stock solution (a liquid) to make a 1% (w/v) solution.4. Top the volume to 100mL by adding 5mL ddH2O from a graduated cylinder.
- Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength.Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength. Answer: You did not give the correct unit. The correct answer is: 333 mL XPenicillin G potassium 5000 units Isotonic Sodium Chloride Solution ad 15 mL Sig. Nose drop Using soluble penicillin tablets, each containing 100,000 units of crystalline penicillin G potassium, explain how you would obtain penicillin G potassium needed in compounding the prescription.