Which of the following could be a reason(s) why a signalling cascade is interrupted, or turned 'off', once it has been turned 'on'?

Human Anatomy & Physiology (11th Edition)
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Cellular signaling follows a cascade of events and has multiple points of regulation. Which of the following could be a reason(s) why a signalling cascade is interrupted, or turned 'off', once it has been turned 'on'?

A) a protein gets tagged with an ubiqutin group and gets degraded

B) a chemical modification occurred on serine, threonine, or tyrosine side chains to turn on/off proteins

C) allosteric regulation causes an active site to be hidden

D) All of the other answers are correct

E) The ligand is prevented from reaching the binding site

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