Question 8 You have performed a peptide conjugation reaction in which the peptide shown below forms four types of products (1-mer, 2-mer, 3-mer and 4-mer). To separate these products you performed a column chromatography in aqueous solution at pH-6. You got the elution profile shown on the plot. a. WHAT KIND OF CHROMATOGRAPHY did you perform and WHY? b. Determine the MONOMER:DIMER MOLAR RATIO in your reaction. EXPLAIN how you arrived at your number. A280 Peptide GSGAGGPGLGGISNAAW 4 3.5 53525150 2.5 1.5 0.5 B C Mii time

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Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.