Which mutation would most likely cause the greatest impact

Using specific examples that have happened in your lifetime, distinguish between a spontaneous mutation and one that is the result of exposure to a substance or radiation.

To determine: The reason due to which scientists think that new genes arise by the duplication of an original gene and divergence by mutation.

Discuss how the DNA can affect the future development of an organism i.e. its physiology and anatomy. You can relate it to the central dogma of biology.