Which antimicrobial agent was most effective against your organism? Can you determine from your results whether this antimicrobial agent will also be most effective against another bacterium? Why or why not? Is this technique measuring bacteriostatic effect, bactericidal effects, or both? Explain.

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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Antibiotic Code Zone of Inhibition (mm) S, I, or R?
Penicillin P 15  
Streptomycin S 8  
Ampicillin A 25  
Chloramphenicol C 14  
Bacitracin B 5  

QUESTIONS:

  1. Which antimicrobial agent was most effective against your organism?
  2. Can you determine from your results whether this antimicrobial agent will also be most effective against another bacterium? Why or why not?
  3. Is this technique measuring bacteriostatic effect, bactericidal effects, or both? Explain.

4) Consider the following list of hypothetical antibiotics, tested against Escherichia coli in a Kirby-Bauer test:

Antibiotic Zone of Inhibition (mm)
Astonostatin 12
Bodaciosporin 25
Crazifloxacin 5
Dorkimycin 16
  1. When the above antibiotics were tested as treatments for E. coli infections in humans, it was found that Dorkimycin has virtually no effect on the infection, while Astonostatin is the most effective of the four antibiotics listed. Propose an explanation for this result.
  2. Based purely on these results, can you draw up a similar table for predicted zones
  3. f inhibition for Staphylococcus aureus? Why or why not?
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