What's the ratio? An acid with a pKapKa of 8.0 is present in a solutionwith a pHPH of 6.0. What is the ratio of the protonated to thedeprotonated form of the acid? e

Acids are hydrogen-containing substances that donate a proton (hydrogen ion or H+) to another…

Answered: What's the ratio? An acid with a pKapKa…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) buffer at pH 7 with 400 mM NaCl. A A purified protein is in a Hepes dialysis membrane tube holds a 5.0 mL sample of the protein solution. The sample tube floats in a beaker containing 0.50 L of the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na*, CI", and Hepes) can to diffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis. Calculate the final concentration of NaCl in the protein sample once the dialysis has come to equilibrium. Calculate the final NaCl concentration in the 5.0 mL protein sample after dialysis in 300 mL of the same Hepes buffer, with 0 mM NaCl, twice in succession. [NaCl] after a single dialysis: [NaCl] after a double dialysis: mM mM
A protein has a dissociation equilibrium constant equal to 4.0um. What is the concentration of free ligand when the fraction of bound sites is 0.7
An enzyme uses a lysine and a histidine as general acid-base catalytic residues. In one direction of the reaction, the lysine and histidine residues are typically protonated (~NH3*, ~Im), but in the other direction of the reaction, the two residues are "reverse protonated" (~NH2, ~Hlm*) (see below scheme). A pH profile of the reaction at various pH values yielded estimates of 7.57 and 9.30 for the macroscopic ionization constants pk,' and pK2, respectively. A separate set of experiments with site-speciffic variants of the enzyme yielded an estimate of 8.78 for the microscopic ionization constant pKp. Given this information, what is the value of the microscopic ionization constant pKA? Report your answer to the nearest hundredth. Lys-NH3* His-HIm" KB H KA H* Lys-NH2 Lys-NH3* His-HIm" His-Im Kc H* Kp Lys-NH2 His-Im
4d
Based on our pka's table, pKa1 of -COOH group of Cys = 1.71 pKa2 of -NH2 group of Cys = 10.78 pKa3 of side chain group of Cys = 8.33 If pKa is different, will the buffer change??
what is the hydrozide ion concentration of a solution made by dissolving 4.5 g of Sr(OH)2 in 500 MI of water
Calculate the mole ratio of sodium acetate and acetic acid needed to make a buffer of p H 4.68 ( see Table 15.1 for Ka values or Appendices G and H for Ka and Ksp values respectively.
so for pH 1, is the net charge 3 as you said up above or 1 down in the solution?
pH of solution 14.00 12.00 10.00 8.00 6.00 First 4.00 equivalence point 2.00 0 First midpoint Second equivalence point Third midpoint pH = pKa=12.32 Third equivalence point HPO4(aq) + OH(aq) PO4(aq) + H2O(1) Second midpoint pH = pKa = 7.21 H2PO4 (aq) + OH(aq) HPO42 (aq) + H2O(1) Using the Henderson- Hasselback equation, show how to create 2L of a 0.1 M KPhos pH 7.5 buffer using K2HPO4 and KH2PO4. The chart to the left should help you understand what pKa to start with. Show your work. pH = pKa = 2.16 H3PO4(aq) + OH(aq) H2PO4(aq) + H2O(l) 25.0 50.0 75.0 100.0 Volume of NaOH added (mL)
The ionizable side change (-OH) of a synthetic amino acid has a pKa of 6.54. Find the amino acid's isoelectric point. The pKa values for the other two are 2.87 and 9.14. ' 65.44% 70.84% 6.00% 4.71%
Example The reference range for blood pH is 7.35 – 7.42. What is this range expressed as [H+] in nmol l-1? Calculation: pH = - log [H+] 7.35 = - log [H+] [H+] = antilog -7.35 = 4.47 x 10-8 mol.l-1 = 44.7nmol.l-1 similarly: pH 7.42 = 38.0 nmol.l-1 Range [H+] = 38 – 44.7 nmol.l-1 If the blood pH decreases in an acidosis from 7.42 to 7.15, what is the change in [H+] in nmol.l-1? so this is example on the worksheet, but i still understand how to answer the question.
An appropriate biochemical buffer should have charges in both its conjugate acid and conjugate base form. This avoids the presence of a neutral form of the buffer that might be able slowly leak into cells. Does unmodified dimethylpiperazine meet this criterion when buffering at pH 8? O Yes No What about buffering at pH 4? D Yes No

SEE MORE QUESTIONS

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS

What's the ratio? An acid with a pKapKa of 8.0 is present in a solution with a pHPH of 6.0. What is the ratio of the protonated to the deprotonated form of the acid? e