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What result do you report for one of your unknown bacteria in terms of mannitol fermentation?
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- Put the following Steps of gram smear in order Create a bacteria Add crystal violet to the bacterial smear. Rinse with water after 60 seconds. Allow the smear to air dry and then heat fix it. Add gram iodine to the smear, let it sit for 60 seconds and rinse water decolorize with 95% ethanolfoe 5-10 seconds and then rise with water Blot dry with bibulous paper Add safranin to the smear and let sit for 60 seconds, then rinse with water Observe using oil immersionWhat is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handleQuestion 6 Describe what the Gram Stain shows: a. The organism is Gram Negative b. The organism is this shape: cocci c. The organism has this arrangement: Question 7
- Microorganism Type of Microorganism Gram Stain Shape Tryptic Soy Agar McConkey Agar Blood Agar EMB Agar Mannitol Salt Agar Bacillus cereus Bacillus megaterium Bacillus subtilis Candida albicans Escherichia coli Micrococcus luteus Neisseria sicca Proteus vulgaris Pseudomonas aeruginosa Serratia marcescens Staphylococcus aureus Staphylococcus epidermidisCreate a short Flow Chart (picture of hand drawn or software generated) with the information you have obtained. Start at the top with Gram Staining Results. Below is a general template (you can delete my example and insert your own) but your flow chart may look different. Just make sure it is clear. Gram Stain Next test here Next test hereLid of petri dish was left on bench top for a few minutes after pouring of sterile medium. Effect: Rationale:
- 1 Figure 3. Two different types of bacterial colonies on an agar plate. Using microbiology terms, describe fully the colonial morphology of the two colonies shown above. A full description will include texture, transparency, color, and form (size, overall shape, margin, and elevation). Colony 1 Colony 2 2.Picture in white is acid fast other picure is negative stain so far this is the information I have: gram stain: positive motility: motile oxygen requirement: Facultative anaerobe and than I need these two acid fast stain: _____??? negative stain: _____????Test Unknown Isolate B 1 Luminescence Positive 2 Cell Shape Straight Rods 3 Gram Staining Negative 4 Oxygen Requirement Facultative Anaerobe 5 Motility (Hanging Drop) Motile 6 Growth at 0% NaCl Negative 7 3% NaCl Positive 8 6 % NaCl Positive 9 Accumulation of PHB Positive 10 Flagella 1 Polar 11 Oxidase Positive 12 Nitrate Reduction 13 Gelatinase Negative 14 Growth at 35OC Positive 15 Growth at 4OC Negative 16 Methyl Red Positive 17 Voges-Proskauer Positive 18 Catalase Positive 19 Starch Hydrolysis Negative 20 Lysine Decarboxylase Negative 21 Indole Production Negative 22 Utilization of 23 Glucose Positive (fermentative) 24 Lactose Positive (fermentative) 25 Mannitol Negative 26 Sucrose…
- The following is a picture of Eosin Methylene Blue Agar. What does the growth on this plate indicate? ASM MicrobeLibrary.org © Cheeptham CGram stain blood bottle what the result shows, what the result means, Thank you!If some cells in your Gram stain are red/pink rods and some are purple cocci, __________. your smear was not made from a pure culture your unknown bacterium is a pleomorphic species the bacteria ferment sucrose your unknown is a species of Escherichia you should inoculate an EMB agar plateSEE MORE QUESTIONS