What is the use and the principle of Clinitest and Benedict’s Test? What is a reducing sugar? Enumerate the different reducing sugars List down the reagents used and procedures of Clinitest and Benedict’s Test
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Urinalysis and Body Fluids
- What is the use and the principle of Clinitest and Benedict’s Test?
- What is a reducing sugar? Enumerate the different reducing sugars
- List down the reagents used and procedures of Clinitest and Benedict’s Test
- How do you interpret the results of Clinitest and Benedict’s Test?
- Describe pass-through phenomenon
- What is the sensitivity and specificity of the two different tests
- What is/are the cause/s of false-positive and negative results of the two different tests?
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- Determination of Creatinine in Serum and Urine Experiment; Question: Why are using this materials/chemicals in this experiment? *10% sodium tungstate *0.75 M NaOH Creatinine depot standard *0.67 N H2SO4 *Picric acid (0.04 M) Procedure: 3 mL of water, 1 mL of 10% sodium tungstate, 2 mL of 0.67 N H2SO4 are added onto the serum (2 mL), mixed well, and filtered after 5-10 minutes (protein-free serum filtrate). 3 mL of this filtrate is used for the test.All tubes are mixed, incubated at room temperature for 20 minutes and read against the reagent blank at 520 nm.First, the serum volume in the test tube is calculated.Since working with 3 mL standard, the absorbance of the standard tube belongs to 30 µg creatinine.Figure 16.9 Urinalysis test. A Chemstrip urine test strip can help determine lness in a patient by detecting substances in the urine. Normal Tests For Results Ogve protein normal Onegative Oegative blood Conclusions: Urinalysis • According to your results, what condition might the patient have? Explain. • Given that the patient's blood contains excess glucose, why is the patient suffering from excessive thirst and urination? • Since neither the liver nor the body cells are taking up glucose, why is the patient tired? • The metabolism of fat can explain the low pH of the urine. Why? 16-15 Laboratory 16 Homeostasis 219To identify a positive control in your lab report, should you list any test tube with positive results? Why or why not? What is a negative control? Which substance is a negative control for each of the tests in this lab exercise?
- What are the commonly used methods for the determination of urea, uric acid, creatinine, creatine, and ammonia in plasma and urine. What interferences might affect the interpretation of the results of these assays and how can they be mitigated? Identify sources of error and variability in these methods and describe the effects on the clinical utility of the laboratory measurements. Kindy insert your references here. Thank you!The reults for the macroscopic part: 0.30M glycerin – solution was translucent (could see text behind the test tube) 0.15M NaCl – solution was opaque (could not see text behind the test tube) 0.30M NaCl – solution was opaque (could not see text behind the test tube) 0.15M glucose – solution was translucent (could see text behind the test tube) 0.30M glucose – solution was opaque (could not see text behind the test tube) 0.30M Urea – solution was translucent (could see text behind the test tube) Results for microscopic part: 0.30M glycerin – no cells present 0.15M NaCl – normal sized cells 0.30M NaCl – crenated (shrunken and star-shaped) cells 0.15M glucose – no cells present 0.30M glucose – normal sized cells 0.30M Urea – no cells present Determine the osmolarity (hypoosmotic, isosmotic, or hyperosmotic) and tonicity (hypotonic, isotonic, hypertonic) of the following solutions.In which solutions did the osmolarity NOT match the tonicity? For those solutions, why did the osmolarity…Paraphrase the text below: Series of test tubes were filled with the desired volume of the BSA (0.1, 0.2, 0.3… 1 ml) .PBS was added to this to make the volume of 1 mL. 5 mL of copper reagent was added to all the tubes. After proper mixing, all the tubes were incubated at room temperature for 15 minutes. 1 mL Folin reagent was added to each and mixed properly with the help of vortex mixer and incubated for 20 minutes. The intensity of the colour was then determined spectrophotometrically at 680 nm. The graph was than plotted between optical density and the amount of BSA. Proteins estimation was done for the detection for the amount of proteins present in the sample solutions by the Lowry method
- By applying Gravimetry, Titration (Chemical indicator) and Potentiometry lessons which technique should be used in following situations. Explain your answer. In your explanation, state what is the advantage of the technique you chose over other technique and /or the barrier/difficulty of using the technique. Your choice should not be dependent on cost. For chem students- Total Acid content per tablet of a newly developed drug that has yellow coating Target level is 1%.What will be the color of saliva extraction with iodine in 3, 6 9, 12, 15, and 18 mins if: you put 5 ml of starch soln. and you heat it to boiling after that you will add 1 ml of saliva. Cool and then continue to heating after that you will place it in a water bath at 60 degree Celsius. At 5 minutes interval for 30 minutes take a drop of the reaction mixture from each test tube and test with Iodine solution (use a spot plate for the test and stir the contents of the test tube before taking a drop). Tabulate the results.6) 1 mL of supernatant is required for a procedure. The final colored solution proves to be too high to read accurately on the spectrophotometer. 100 μL of supernatant and 900 μL of distilled water are substituted for the original supernatant and the procedure, run as before. The reading from the standard curve is 46 mg/dL. What is the actual amount of substance in the patient serum?
- In detection of Albumin in urine, what are the mechanisms, reactions, possible results and interpretation involve in Sulphosalicylic acid test and Heller's testThe first step of a urinalysis procedure, before testing with a reagent stick, is to visually examine the urine for color, clarity and odor. These properties should be considered in addition to a patient’s clinical presentation, fluid intake and urine output. In the case of Sample B, the patient has presented with an overall healthy appearance. The quantity of urine is normal, the clarity of the urine is clear, but the color of the urine is tinged red, the odor is strong, and the patient is alarmed. From the following, select ALL questions which the patient should be asked, to help determine the cause of the color and odor changes. a. menstruation should be ruled out in females b. consumption of beets c. consumption of asparagus d. consumption of strawberriesExperiment No. 6 Milk.pdf - Adobe Acrobat Reader DC File Edit View Sign Window Help Home Tools Experiment No. 6 .. x 玩, 2 14 116% 3. Formation and Composition of Film from Milk a. Place 10 mL of fresh milk in an evaporating dish. b. Boil and observe the formation of film. Observation: c. Remove the film and then boil again. Observation: d. Test the film with Biuret's test and Acrolein test. Search Signa Convert E Edit PDF Lo Create PDF Test Performed Result with Milk Film Substance present Biuret's test Adobe PDF Pack Acrolein test Convert files to PDF anc easily combine them wit other file types Guide Questions: 1. What can you conclude regarding the chemical composition of the film formed? Select File to Convert to P e. Get 10 mL fresh milk, then acidify it with acetic acid, and repeat the above experiment. Select Fite 2. Did you get a film on boiling sour milk? 3. Explain the mechanism involved in the formation of film. Create, edit and sign PDF forms & agreements Start Free Faf B.…
