What is the role of the DNA Polymerase I 5' --> 3' exonuclease activity at a replication fork? O remove RNA primers fill in gaps after primer removal
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- During DNA replication, the function of RNA primers is to serve as a binding site for DNA ligase open replication bubbles O serve as starting points for DNA strand elongation by DNA polymerase I in the 3' - 5' direction as starting points for DNA strand elongation by DNA polymerase III in ser prokaryotes prevent new-separated strands of DNA from rejoiningDuring DNA replication, one of the new strands of DNA is synthesized continuously, while the other is synthesized as a number of separate fragments of DNA that are subsequently linked by DNA ligase. This is because O replication starts at many points on the chromosome RNA primers only anneal to one of the parental strands of DNA one of the parental strands is unwound slower than the other by helicase DNA polymerase III only synthesizes DNA in the 5' - 3' directionHelp me please
- If primase makes an error and incorporates an incorrect base in the nucleic acid primer, then why would this not be a problem given the DNA replication mechanism? O Primase only copies end sequences that will be protected by telomers The lagging strand utilizes a helicase to prevent replication errors O Primase can be error proofed using a domain of DNA polymerase O The RNA primer sequence is removed and not used as a templateConsidening the structure of DNA, what kind of bonds hold one nucleotide to another within one side of the DNA molecule? O hydrogen O phosophodiester O lonic peptideDescribe precisely the catalytic activity of DNA polymerase I that removes RNA primers. 3' to 5' exonuclease 5' to 3' exonuclease 5' to 3' endonuclease O 3' to 5' endonuclease
- What does i mean to say that extension by DNA polymerase III proceeds 5' 3'? The 5' end of a DNA polymerase molecule attaches to the 3' end of primase. DNA polymerase adds nucleotides to a growing strand, moving in the 5'-3' direction. O DNA polymerase seals nicks as it moves along a DNA strand toward the 3' end. DNA polymerase can only synthesize DNA at the 5' end of an existing strand of DNA. O O 0Match the enzyme on the left with its role in DNA replication DNA polymerase I helicase DNA ligase DNA polymerase III topoisomerase primase 72 W w# 3 E $ 4 R % 5 T A 6 MacBook Pro Y & 7 U * 8 replaces primers with DNA connects Okazaki fragments to form a continuous strand of DNA synthesizes short RNA fragments used to initiate DNA synthesis Uses the 3'OH of an RNA primer to synthesize the leading strand and Okazaki fragments keeps DNA from getting tangled up ahead of the replication fork "unwinds" the DNA double helix at the origin and replication forks 1 ( 9 X 0 0 P + 11 NextGiven that mammalian cells have much larger chromosomes compared to E. coli cells, how is replication in the former carried out rapidly? O Eukaryotic DNA polymerases are extremely fast compared with prokaryotic polymerases O Multiple replication forks work simultaneously on each chromosomal DNA O The higher temperature of mammalian cells allows for an exponentially higher replication rate O The presence of histones speeds up the rate of chromosome replication
- DNA synthesis by DNA polymerases exemplitiės Q6 DNA synthesis Growing strand Template (primer) strand DNA polymerase G NH DNA primer Mg2+ NH2 Asp- Asp- 5' P-0 3' OH OH Mg2+ Incoming DNTP H. Incoming DNTP 0-P=0 Mg H. Mg CH H. Can you' rationalize or explain why dNTP (deoxyribonucleotide triphosphate) instead of DNMP (deoxyribonucleotide monophosphate) has been selected by evolution as the reactant and a DNA polymerase is also required to catalyze the reaction (to a reasonable rate of ~1000-events per second) in living systems? Hint: Ea of the reaction without catalysis is about 30 kcal/mol.Which of the following statement(s) is/are false/incorrect? You may select multiple options, if necessary. ☐ Experiments support bidirectional movement of multiple replication forks at eukaryotic chromosomes. O Mg++ ions play important roles during DNA synthesis Synthesis of DNA is semi-conservative ✔ Beta clamp reduces DNA polymerase processivity ✔ DNA polymerase III uses ATP as a source of energy to drive the polymerization reaction forward O Tautomers enhance chances of correct nucleotide incorporation during DNA synthesisDuring DNA replication, the function of RNA primers is to Group of answer choices serve as a binding site for DNA ligase separate the two strands of the double helix to open replication "bubbles" serve as starting points for DNA strand elongation by DNA polymerase in the 3' - 5' direction prevent new-separated strands of DNA from rejoining serve as starting points for DNA strand elongation in the 5' - 3' direction by DNA polymerase
