If an esculinase-postive and an esculinase-negative bacteria species can both grow on the Bile Esculin Agar media, how are the two bacteria species distinguished?
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If an esculinase-postive and an esculinase-negative bacteria species can both grow on the Bile Esculin Agar media, how are the two bacteria species distinguished?
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- Vibrio cholerae can be cultured on Thiosulfate-Citrate-Bile salts-Sucrose agar (TCBS). Enterobacteriaceae and gram-positive bacteria are inhibited on this agar. cholerae produces large yellow colonies while other Vibrio species produce blue, green, or blue-green colonies. Discuss the purpose of each media listed. Based on this information alone, what term(s) would best describe this agar? Selective Enrichment (enriched) Differential Reducing Selective and enriched Selective and differential Selective, differential, and enrichedClostridium spp palys vital role in meat spoilage. a) Describe possible conditions of spoilage cause by this bacterium b) suggest factors that can contribute to spoilage condition stated in (a). c) suggest possible process that can be done to avoid the growth of this bacteriaIn the Mannitol Salt Agar, why is there no observed growth of Escherichia coli?
- What is the mechanism of the bile esculin test?Not only is blood agar an enriched medium that can support fastidious organism growth, but it can also serve as a differential medium in the identification of streptococcal species because of the presence of red blood cells that can be destroyed by an organism's hemolysins. Looking at this photo, how could you describe the organism growing on the surface of this blood agar plate? Multiple Choice a) Gamma-hemolytic b) Alpha-hemolytic c) Non-hemolytic d) Beta-hemolyticHow would you interpret the following result of the Bile Esculin Test: "Growth of the bacteria is present however no darkening of the agar can be observed after 48 hours." Please explain.
- How does bile in bile esculin medium contibute to the isolation and presumptive identification of Enterococcus?Is the Mueller-Hinton Agar (MHA) a complex or defined medium? Explain based on its composition. Is MHA a A) differential, B) selective, C) both differential and selective media, or D) neither? Explain based on what kind of microorganisms it allows to grow.How do the methyl-red and Voges-Proskauer tests differentiate between certain members of the Enterobacteriaceae?
- The isolation of Salmonella Typhimurium and S.Typhi require different protocols: A) In what aspects do these protocols differ? B) why do these protocols differ? C) How will you be able to distinguish between these organisms on XLD and Hektoen Agar?Can lactic acid culture infect the human gastrointestinal tract? If yes, why is this method not used to treat lactose-intolerant individuals?How would you distinguish between Listeria monocytogenes and othe Listeria species on Chromogenic Listeria agar supplemented with Brilliance Listeria Differential supplement (SR0228)?