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- Ex. 34 = Indole Production from the Amino Acid Tryptophan and Catabolite Repression (p. 291) What is catabolite repression? How does it work? What do negative/positive results look like and what do they tell you about bacterial metabolism? What is tryptophan and how does it relate to indole? Ex. 33 = Microbial Hydrogen Sulfide (H2S) Production from Thiosulfate and Sulfur-Containing Amino Acids (p. 285) How is sulfate used in bacterial metabolism? How can this be detected in the laboratory? What do negative/positive results look like and what do they tell you about bacterial metabolism? What is the role of peptone in the agar?Which amino acid is an ideal substrate for the oxidative deamination reaction?(a) What are the Vmax and Km (including units) for Tigerzyme? b) What are the Vmax and Km (including units) for Tigerzyme in the presenceof the inhibitor Tar Heel? (c) What are the Vmax and Km (including units) for Tigerzyme in the presenceof the inhibitor Blue Devil?