How is the specified substrate determined?
Q: What is meant by “salting out”? How does it work?
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A:
Q: Which organism cause kala-azar in humans?
A: Visceral leishmaniasis is commonly referred to as kala-azar. It is caused by the protozoan parasites…
Q: What materials can easily diffuse through the lipidbilayer, and why?
A: The lipid bilayer is one of the components of the cell membrane. This polar membrane manages the…
Q: How is hemolytic anemia related to pentose phosphate?
A: Introduction: It is anemia due to hemolysis of red blood cells that occurs either in the blood…
Q: How can the specificity ofSubstrate Concentration be determined?
A: Specificity of an enzyme to its substrate is how efficient an enzyme is in choosing its right…
Q: What is the purpose of the Frank-Starling mechanism?
A: The heart can be defined as the muscular organ that is approximately about the size of a fist and it…
Q: Explain what is Phenylketonuria (PKU) ? How it affects the body ?
A: Phenylketonuria, also known as PKU, is a rare inherited disorder that causes an amino acid called…
Q: What criteria are used to determine the specified substrate?
A: An enzyme is a biological molecule that works as a catalyst in living organisms, controlling the…
Q: What is the mechanism of the reaction of the Bradford reagent with proteins? (explain in not less…
A: The Bradford protein assay is used to measure the concentration of total protein in a sample.
Q: What are the modern techniques used to identify the active sites of an enzyme?
A: The part of the enzyme where the substrate binds is called the active site. An enzyme molecule has…
Q: What is the Haber process?
A: The Haber process is also called as the Haber-Bosch process or Haber ammonia process.
Q: Is the product SARDINES is affected by protein denaturation during processing and storage? If so, is…
A: Protein denaturation is a concern in fisheries products. As a result, additional verification tests…
Q: How is galic acid obtained?
A: Gallic acid is a trihydroxy benzoic acid, which is a type of phenolic acid found in gallnuts, sumac,…
Q: explain the phenylketonuria disease mechanism
A: Inherited diseases are those diseases that come from parents to offspring via the genetic route,…
Q: Why are proteins purified? Explain with examples
A: Protein purification is a series of processes intended to isolate a single type of protein from a…
Q: Can the TCA Cycle Provide Intermediatesfor Biosynthesis?
A: TCA cycle is the final common oxidative pathway for fats, carbs and amino acids. The enzymes of TCA…
Q: What common substance inactivates nitrogenase enzyme by binding to its active site?
A: Microbial cells carry out the fixation of atmospheric nitrogen and convert it into ammonia. The…
Q: Define the deadenylases ?
A: Enzymes are protein molecules which acts as a catalyst and speed up the reaction when they act on…
Q: What amino acids are obtained from the same metabolic intermediates when the amino acids are…
A: There are 20 amino acids formed during the translation process, which involves conversion of…
Q: What is application of D_serine?
A: Amino acids are the building blocks of proteins where the combination of 20 different proteins helps…
Q: what substances interfere with the tests for fructose? for lactose?
A: Seliwanoff's test is the qualitative test for the detection of ketose sugars in a sample. The ketose…
Q: What is CHCA ?
A: It's a chemical compound.
Q: Explain the mechanism of urease activity
A: The urease is an enzyme that is used as a microbiological indicator to distinguish the…
Q: What are the properties of Tryptophan?
A: Tryptophan is an essential amino acid that consists of an alpha-amino group. alpha carboxyl group…
Q: How do you determine protein concentration using bicinchoninic acid assay?
A: Proteins are polymers that are composed of amino acids. Amino acids are in turn made up of a central…
Q: Source of Alanine transaminase?
A: Introduction: The enzyme alanine transaminase (ALT) is a transaminase. It's also known as alanine…
Q: Explain the extraction method and the procedure for extraction of Echinolone by using…
A: Chromatography works on the principle of molecules in a mixture being applied to a surface or a…
Q: What is TCA? What are the different enzymes involved in TCA? What are the important products in…
A: Cellular respiration is a series of metabolic reactions and activities that occur in organisms'…
Q: What is a lactose fermenting E.coli ( LFEC) ?
A: E.coli is a gram negative rod shaped bacterium. It undergoes lactose fermentation to produce lactic…
Q: Instead of Molisch's test, what specific test can be used to detect the presence of pentose sugar?
A: A monosaccharide that has five carbon atoms is referred to as pentose sugar. Ribose present in RNA…
Q: Why Cepacol is used ?
A: Cepacol is a drug which relieves pain associated with a sore throat or mouth. It can be administered…
Q: What will be the products? Which enzyme will catalyzes this reaction?
A: Proteases are synthesized as inactive proteins called zymogens. They are cleaved by other proteases…
Q: What major biosynthetic reactions utilize PRPP?
A: PRPP is an important intermediate in cellular metabolism. It is synthesised by PRPP synthase, as…
Q: Why must protein degradation be somewhat selective?
A: Proteins in the cells perform several functions such as some acts as enzymes, transcription factors,…
Q: What is the basic strategy by which amino acids are degraded?
A: Amino acids are biomolecules that join together to form proteins. Amino acids are the structural…
Q: cal is utilized as the reagent known as VP-B?
A: The Voges-Proskauer (VP) test is generally used to test if an organism produces acetyl methyl…
Q: Would the ninhydrin test useful and reliable for showing the presence or absence of proteins?
A: Proteins are the most extensive class of biological macromolecules in terms of chemical and…
Q: How does the nucleic acid being purified from associated protein and other impurities?
A: Nucleic acids isolation and purification involve lysis of the cells followed by the removal of…
Q: What are some environmental conditions that must be controlled while purifying a protein?
A: Protein purification is a vital step in biochemistry. It is important for the characterization of…
Q: What are the identities and functions of the components of the Bradford reagent in protein content…
A: Different quantitative assays for the proteins are as follows: Biuret method Lowry's method…
Q: Differentiate sweet molecules in Column A using the criteria in column B.
A: Acesulfame - K:- It is an artificial sweetener that is calorie-free, mostly used in sugar-free…
Q: What amino acid present in proteins is responsible for the reaction in Hopkins- Cole test ?
A: Amino acids are the monomer of the protein. Each amino acid has a carboxyl group, an amino group, a…
Q: what is the present enzyme in making kabocha squash soup?
A: Kobocha squash soup is Japanese variety of winter squash.its Having heavy and thick-fleshed texture…
Q: How do the critical amino acids catalyze the chymotrypsin reaction?
A: Chymotrypsin is synthesized in the pancreas as a precursor called chymotrypsinogen that is…
Q: Is there a difference between the effects of sugar concentrations on protein denaturation? Why?
A: Protein denaturation: It is the process when the secondary, tertiary, and quaternary structure of a…
Q: What is the Mechanism of lysozyme catalysed by enzymes without cofactors?
A:
How is the specified substrate determined?
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- Propose reagents for the conversion of (B) to (C).Is carbonic anhydrase a metalloprotease? If so, what is the mechanism for the enzyme and a proposed substrate?Serum blood of a patient with dislipoproteinemia type 1 has milky appearance even in fasting. If serum stays at low temperature (40) for several hours fatty layer appears on its surface. What are the possible causes of these symptoms? To explain this, answer the questions and do the following tasks: a) what compounds of serum must be tested for that patient in biochemical lab? b) write the reaction which does not occur properly in patient’s blood; c) write down the schemes, explaining how the products of the previous reaction are used in adipose tissue and heart in healthy person 2 hours after a meal.
- what is an enymeWhich amino acid is an ideal substrate for the oxidative deamination reaction?Ex. 33 = Microbial Hydrogen Sulfide (H2S) Production from Thiosulfate and Sulfur-Containing Amino Acids (p. 285) How is sulfate used in bacterial metabolism? How can this be detected in the laboratory? What do negative/positive results look like and what do they tell you about bacterial metabolism? What is the role of peptone in the agar?
- What are the major coenzymes? Briefly describe thefunction of each.What is the medical diagnostic value associated with the presence of the following enzyme in the bloodstream? ALTAn enzymatic reaction was carried out in a batch-operated reactor until exhaustion of the substrate. The formation of the product was monitored over time, obtaining the results shown in Table 1. Answer: a) Knowing that the initial substrate concentration was 0.2 M and that the stoichiometry reaction is: 1S –> 2P, estimate the substrate concentration over time from the data from [P] data in Table 1; b) Determine the parameters of the Michaelis-Menten equation, Vmax and Km, by fitting direct from the hyperbola function; c) Determine the parameters Vmáx and Km, by the linearized equation;