What is the difference between complete medium and minimal medium? How are complete media and minimal media to which one or more nutrients have been added (selective media) used to isolate auxotrophic mutants of bacteria?

You have several different media onto which you inoculated eight strains of yeast (A-H). The media include a rich medium, an unsupplemented minimal medium, and minimal media each supplemented with one vitamin. Of the yeast strains, one is a prototroph and seven are auxotrophs for a vitamin. After overnight incubation, the following results were observed (tan patches represent growth): D plate 1 (A) B DE F GH plate 5 plate 4 plate 6 Which plate contains an unsupplemented minimal medium? [Select] Which plate contains a rich medium? [Select] plate 2 Which strain is a prototroph? [Select] Strain E is an auxotroph for niacin. Which plate reveals this specific auxotrophy? [ Select] plate 3 plate 7 One strain is an auxotroph for both choline and pantothenic acid. Which one is this most likely to be? [Select]

Describe how Blue/White screening reagent helps in screening the colonies of transformed cells.

How do you recognize individual colonies? Describe color, shape, size. Explain how the streak plate method enabled you to identify a single species from a mixed culture?

Do Gram-positive bacteria grow on PEA agar? If not, what prevents their growth?

How can single-cell genomics be used to addressmicrobial dark matter?

With regard to studying the mechanism of conjugation,what is the purpose of using a U-tube?

Why is it necessary to inoculate the KIA medium all the way down to the bottom of the tube during inoculation?

E. coli O157:H7 is an organism of concern in contaminated foods. How can the use of MacConkey agar and Sorbitol-MacConkey agar be used to screen for E. coli bacteria in general and E. coli O157:H7 in particular?

What is the structural and functional differences between Inoculating loop versus inoculating needle?