What is the structural and functional differences between Inoculating loop versus inoculating needle?

Q: What are the advantages and disadvantages of using fi ltration as a method of control?

A: Filtration is the separating of substances based on their different chemical and physical qualities.…

Q: You want to set up a 1:10 dilution series, so that you can plate a 10-1, 10-2, and a 10-3dilution;…

A: It is basically done for counting the  microorganisms like viruses, bacteria etc  from an unknown…

Q: What is Xanthan ? What is the importance of using Xanthan in the emulsion experiment?

A: Biopolymers are polymers, mainly polysaccharides, which are produced by microbes. They are mainly…

Q: What is Circular Dichroism? Discuss its theory and applications.

A: Circular dichroism is a form of light absorption spectroscopy. This method was first described by…

Q: Lucas's unwashed, dirty hand produces 947 CFU when swabbed onto a nutrient agar plate, while his…

A: CFU stands for colony forming unit.

Q: Why do we need a standard dispensing process?

A: The standard dispensing process is a standard operating procedure (SOP) in the pharmacy practice…

Q: what molecules can prevent anabolic steriods form working?

A: It acts by promoting nitrogen retention in muscles, leading to an increase in muscle size, and…

Q: 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes…

A: Calibration graphs are used to estimate the concentration of unknown sample by plotting it against…

Q: Penicillin heralded the dawn of the antibiotic age when it was discovered in 1928 and is now the…

A: Penicillin is now widely used antibiotic and hence its accurate concentration determination is…

Q: What is the purpose of using the Z-track technique for IM injections.

A: Select and Prepare the Site :Choose an appropriate injection site commonly gluteal and deltoid) and…

Q: What is the purpose of the negative and positive control in the Disc diffusion assay? Why is it…

A: The disc diffusion assay is used to determine the susceptibility of test bacteria to different…

Q: Define aseptic technique and briefly explain what we should do in the lab to provide aseptic…

A: The main aim of aseptic technique is to maintain a sterilized area in the lab and to control spread…

Q: What is the purpose of the TSI test?

A: A triple sugar iron test or TSI test is used to test a microorganism's ability to ferment sugar and…

Q: -Two hundred patients without chest pain were screened with a new biochemical test that showed 190…

A: A screening test is important in the diagnosis of any disease at the initial stage. In some cases,…

Q: What is the difference between normal and reverse phase chromatography?

A: The main Difference between Normal and reverse phase chromatography is 1) In normal phase…

Q: How does gel filtration work? What are the applications of analytical versus preparative versions…

A: Chromatography is a technique for separating a mixture in the laboratory. The mixture is dissolved…

Q: What is the procedure of beta method?

A: Beta-lactamase test is used for the detection of enzyme beta-lactamase. This enzyme provides the…

Q: In the assay of citric acid, what do you think about the nature of solution sample ? a) Before…

A: In the citric acid assay, the concentration of the citric acid is used to determine with the help of…

Q: For solid dosage form what are the SOP that we must follow during dispensing process?

A: Solid dosage form includes tablets and capsules. SOP (Standard Operating Procedure) is a collection…

Q: How should you prepare your running buffer?

A: SDS-PAGE is method of separation of protein based on its size. Running buffer of the SDS-PAGE…

Q: 7) One of the following antibiotics doesn’t work through the same class of molecules as the rest: a)…

A: Daptomycin works through a different class of molecules known as lipopeptides, whereas the rest of…

Q: List three biomaterials that are used in cancer diagnosis and therapeutic. b) List three…

A: Cancer, also called malignancy, is an abnormal growth of cells. There are more than 100 types of…

Q: If a technician examined a Kirby-Bauer assay and found individualcolonies of the plated microbe…

A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…

Q: Describe two different applications of superparamagnetic particles in bio-medicines and what are the…

A: Due to its special characteristics, superparamagnetic nanoparticles or superparamagnetic particles,…

Q: What is optical density (OD) and how is it measured? How is it related to the concentration of the…

A: UV–vis spectrophotometric optical density (OD) is the most commonly-used technique for estimating…

Q: What is the difference and compare the light source and path of light of a immunofluorescence…

A: Introduction  : A microscope is a tool that can be used to examine cells and other tiny objects.…

Q: Explain how to conduct a Kirby-Bauer disk diffusion test. Include all of the steps involved

A: INTRODUCTION Kirby - Bauer Disc diffusion method Kirby - bauer disc diffusion method is widely known…

Q: What is the role of ethidium bromide in the procedure and why do other scientists find alternatives…

A: INTRODUCTION Agarose gel electrophoresis This is a type of gel electrophoresis used for the…

Q: What is the principlels of in-dose test?

A: During the development of drugs, the efficacy of a new drug is typically determined by showing its…

Q: A phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to…

A: Phagehunter is the study of unknown bacteriophages with the knowledge of microbiology, molecular…

Q: Nanoparticles were added in a liquid to prompt the medication release, and it was observed that 5 mg…

A: Medication: Medication refers to any substance or drug that is used to treat, cure, prevent, or…

Q: Besides agarosc gel electrophoresis, there is also macromolccules. Differentiate both these methods.…

A: Agarose gel electrophoresis and SDS-PAGE are techniques for separation of macromolecules.

Q: c) In performing an immunological procedure, you add 2 mL of stock serum to 8 mL of saline. Two 1/4…

A: We are adding 2 ml of serum to 8 ml of saline, then the total volume will be 10 ml. Dilution factor…

Q: What are the four steps of an ELISA (enzyme-linked immunosorbent assay) protocol?

A: The objective of the question is to understand the four main steps involved in an ELISA…

Q: With an A260 value of 1.12 and a DNA concentration of 56.0 ng/mL, what is the pathlength of the…

A: The Beer-Lambert Law must be applied in order to determine the pathlength of the optics utilised…

Q: How does concentration of the buffer affect current in electrophoresis? What are the implications on…

A: In electrophoresis if electric current is applied to ions of a medium then the ions will move within…

Q: Outline in detail the procedure for celloidin embedding .

A: Embedding is that the method during which the tissues or the specimens are capsulate in a very mass…

Q: w

A: In NGS, GC-poor or GC-rich sequences, lead to an uneven coverage or even no coverage of reads across…

Q: How can you make a final dilution of 10-10  using 5 dilution tubes? Outline your steps.

A: Dilution is generally done to decrease the concentration of a solute in a solvent.

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.