What is the structural and functional differences between Inoculating loop versus inoculating needle?

Q: Ex. 5-4 Catalase 1) In the Catalase test (5-4), if bacteria was added to hydrogen peroxide on the…

A: The study of tiny living creatures such as bacteria, viruses, fungus, algae, and protozoa is known…

Q: Why should the cell density of the inoculum be as small as possible? Why can we not standardize the…

A: The process of introduction of microbes into a nutrient media or other similar environments which…

Q: h) Acacia is rarely used as a tablet binder because it can encourage a) microbial proliferation b)…

A: These are some multiple choice questions.

Q: Why do we need a standard dispensing process?

A: The standard dispensing process is a standard operating procedure (SOP) in the pharmacy practice…

Q: Penicillin heralded the dawn of the antibiotic age when it was discovered in 1928 and is now the…

A: Penicillin is now widely used antibiotic and hence its accurate concentration determination is…

Q: Refer to the diagram below of some of the key components found inside an agarose gel electrophoresis…

A: when an electric current is applied to the gel, DNA will migrate towards the positively charged…

Q: how is the streak plate technique used to isolate and purify psychrotrophic spoilage-causing…

A: Microbiology is an indispensable subject of biological sciences that concerns the study of microbes…

Q: Discuss the role of the addition of ammonium chloride potassium lysius buffer in splenocyte culture…

A: Any form of white blood cell that is found in the spleen or has been separated from splenic tissue…

Q: What is the purpose of the negative and positive control in the Disc diffusion assay? Why is it…

A: The disc diffusion assay is used to determine the susceptibility of test bacteria to different…

Q: Define aseptic technique and briefly explain what we should do in the lab to provide aseptic…

A: The main aim of aseptic technique is to maintain a sterilized area in the lab and to control spread…

Q: A phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to…

A: High titer lysate:            A concentrated liquid sample of phage obtained by the infection of the…

Q: -Two hundred patients without chest pain were screened with a new biochemical test that showed 190…

A: A screening test is important in the diagnosis of any disease at the initial stage. In some cases,…

Q: During a flame test, why is it necessary to use a new splint for each element?

A: A splint is a thin wood/stick used commonly in laboratory for performing a flame test. The splint is…

Q: what is the purpose of the FeCl3 in this aspirin test?

A: Now, setting up and observing the breakdown:1) Divided: We split apirin into two tubes, one with HCl…

Q: In the Avery, McLeod, McCarty Experiment where supernatant from heat killed, virulent S Strain…

A: In this talk, we will learn about a very important experiment called the Avery, McLeod, and McCarty…

Q: In step 3 of today's protocol, it mentions that the incubation step with the P2 buffer should not be…

A: P2 buffer is a lysis buffer solution containing sodium dodecyl sulfate to puncture holes in cellular…

Q: In the amino acid decarboxylase test (lysine decarboxylase), we added mineral oil to the tube before…

A: Introduction The Lysine Decarboxylase Test Is Based On Some Bacteria's Capacity To Convert L-lysine…

Q: How does gel filtration work? What are the applications of analytical versus preparative versions…

A: Chromatography is a technique for separating a mixture in the laboratory. The mixture is dissolved…

Q: What other method could be used to purify lysozyme from white using its unusual pl?

A: The unique charge properties of lysozyme with a very high pI of about 10.5 can be used in the…

Q: Biochemical and differential tests can be incorporated into growth media or performed on isolated…

A: It is required to provide three examples of differential test that is incorporated into growth media…

Q: For solid dosage form what are the SOP that we must follow during dispensing process?

A: Solid dosage form includes tablets and capsules. SOP (Standard Operating Procedure) is a collection…

Q: Why must materials and instruments used in tissue culture be kept sterile?

A: Sterilization of explants is a key step in plant tissue culture.

Q: 7) One of the following antibiotics doesn’t work through the same class of molecules as the rest: a)…

A: Daptomycin works through a different class of molecules known as lipopeptides, whereas the rest of…

Q: Complete the following table summarising the neutral red assay data from the group following…

A: Cell Viability Assays -- It is a measure of the proportion of healthy , live cells with in a…

Q: List three biomaterials that are used in cancer diagnosis and therapeutic. b) List three…

A: Cancer, also called malignancy, is an abnormal growth of cells. There are more than 100 types of…

Q: Describe two different applications of superparamagnetic particles in bio-medicines and what are the…

A: Due to its special characteristics, superparamagnetic nanoparticles or superparamagnetic particles,…

Q: A phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to…

A: Phagehunter is the study of unknown bacteriophages with the knowledge of microbiology, molecular…

Q: c) In performing an immunological procedure, you add 2 mL of stock serum to 8 mL of saline. Two 1/4…

A: We are adding 2 ml of serum to 8 ml of saline, then the total volume will be 10 ml. Dilution factor…

Q: What is the advantage of cryo-sectioning for antibody staining methods?

A: To study the antigenic structures, present on the cell surface tissues can be embedded in paraffin…

Q: How does concentration of the buffer affect current in electrophoresis? What are the implications on…

A: In electrophoresis if electric current is applied to ions of a medium then the ions will move within…

Q: What is the procedure for methylene blue stain on e.coli?

A: Methylene Blue is a cationic stain, which binds to negatively charged parts of the cells, such as…

Q: Can a P1000 micropipette be used to accurately pipet a glycerol solution? Why or why not? What about…

A: We must obtain accurate and exact measurements in order to achieve adequate and effective…

Q: List the mechanism and give several names of the following: Class I, Class II, Class III, and Class…

A:

Q: The following questions refer to the analysis of cells using flow  cytometry  A) When analyzing…

A: Flow cytometry is a common cell biology technique using laser-based technologies in a heterogeneous…

Q: A flow cytometer is constructed of many components. For good quantitative results how and how…

A: Flow cytometry is a powerful technique used in various fields of science and medicine to analyze and…

Q: cal is utilized as the reagent known as VP-B?

A: The Voges-Proskauer (VP) test is generally used to test if an organism produces acetyl methyl…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.