What is the biochemical basis for the spectrum of fastidiousness seen in the microbial world? (That is, why are some organisms fastidious and others are non fastidious?)

Example 14.6 The oxygen requirement for cell growth in glueose can be represented by the following equation (Mateles, 1971) 32NC+8(Nh2)+16(No2) Fx/sM +yo2 – 2.67yc + 1.714YN2 – 8y H2 ro2 = In which ro2 is the oxygen required for each gram of cells produced, N stands for no. of atoms present in each molecule substrate, y stands for mass fractions and M is the MW of the substrate. The yeast cell may be considered to be CH1.800.5NO.2. Calculate rO2 if the yield factor (Yx/s) is 0.46g of cells produced for each gram of substrate consumed.

A bacterial culture is grown using either octadecane (C18H38) or pentachlorophenol (C6HCl5O) as the sole source of carbon and energy. The cell yield value is determined by dry weight analysis to be 1.49 for octadecane and 0.05 for pentachlorophenol. Using either octadecane or pentachlorophenol, please describe the steps taken (and the final result) showing what percentage of the substrate carbon will be found as cell mass and as CO2?  Please show all calculations.

From the choices provided below, list the reagent(s) in order that will yield the following transformation. Br

The molecular iodine (12) in betadine is susceptible to decomposition if exposed to direct sunlight, which lowers the concentration of 12 solution. After performing the standardization step in home experiment 9, a student leaves their betadine solution in direct sunlight for several hours before coming back to perform the titration of ascorbic acid in orange juice. Assuming that the concentration of 12 is reduced by the sunlight, how would this affect the measured value of the concentration of ascorbic acid in the orange juice? The measured concentration of ascorbic acid would be higher The measured concentration of ascorbic acid would be lower There is not enough information to determine the affect on the measured concentration of ascorbic acid The measured concentration of ascorbic acid would be unaffected

Is the formation of a serine ester an example of acid/base catalysis, group transfer or electrostatic stabliziation?

How is it possible to determine the structure of an enzyme substrate complex by x ray crystallography when the reaction is over so quickly and the x ray analysis takes at least several minutes?

Following is a Dixon plot for PNPP inhibition by inorganic phosphates. What answer choice shows the correct combination of the mode of inhibition and Ki of the EI complex?   - Mixed inhibition, Ki= -21.2 uM - Mixed inhibition, Ki= 1/16.5 uM - Competitive inhibition, Ki= 16.5 uM - Competitive inhibition , Ki= -1/16.5 uM - None of the above

Chymotrypsin is an enzyme that is optimized to function in the small intestine (at a pH of ~7.4). Explain, given what you know about the chymotrypsin mechanism and what we learned about acid/base chemistry, why chymotrypsin would not be able to initiate catalysis if it were secreted into the stomach (pH ~2.5).

What role do low-barrier hydrogen bonds play in serine protease catalysis?