What is the Ames test and how is it carried out? What assumption concerning mutagenicity and carcinogenicity is it based upon?

Q: What is a mutagen? Name two common mutagens andtheir effects on DNA

A: The cells are basic structural and functional units of life. The human body contains many types of…

Q: What is a forward genetic screen?

A: Genetics is a division of biology that includes study of heredity, genes and genetic variation.…

Q: Describe the molecular genetics process using proper scientific terminology. Describe the steps that…

A: Molecular genetics has been proved to be revolutionary. From producing bulk drugs of animal origin…

Q: What is a simple way of making a knockout mouse in terms of DNA recombinant technology?

A: A knockout mouse, or knock-out mouse, is a genetically modified mouse in which researchers have…

Q: What is central dogma of molecular genetics? How does it work ?

A: The Central Dogma was first proposed in 1958 by Francis Crick. He was also the discoverer of the…

Q: discuss what is mutagenesis and what mechanisms are invloved?

A: Mutagenesis : It is the process by which an organism's deoxyribonucleic acid change , resulting in a…

Q: Describe the following techniques for determining gene or protein function with advantages…

A:

Q: Describe how the Ames test screens for potential environmental mutagens. Why is it thought that a…

A: Ames test is a biological assay to estimate the ability of a chemical compound to cause mutation.…

Q: What vectors can be used for cloning?

A: Introduction: Vectors are the molecular vehicles used for the transfer of genes of interest into the…

Q: What is the difference between nonhomologous end-joining (NHEJ) and homology-directed repair (HDR)…

A: Genome editing can be defined as the way of of making  the specific changes to the DNA of a cell or…

Q: What is meant by Genetic Information NondiscriminationAct (GINA) ?

A: Genetics can be defined as the branch of biology which is concerned with the study of genes, genetic…

Q: How can we estimate the age of a mutation?

A: Mutation is defined as sudden inheritable change that occurs in the DNA sequence. Mutation occurs…

Q: What activities does the Genetic Information Nondiscrimination Act prohibit?

A: The Genetic information non-discrimination act (2008) is a law that defend the individuals from the…

Q: List three possible uses of site-directed mutagenesis

A: BASIC INFORMATION MUTATION  It is sudden or discontinuous variation These changes occurs in the…

Q: Genetic engineers would like to insert the gene for producing thrombin, a protein that facilitates…

A: Genetic engineering is the process of modifying an organism's genetic makeup using recombinant DNA…

Q: Can the Ames test detect all mutagenic chemicals? What application does ithave in the pharmaceutical…

A: Biology is the scientific approach to study life. All the living forms share certain key features…

Q: What is a transversion mutation?

A: Mutations are the alterations or the changes that occur in the DNA. Mutations are variations in…

Q: What is the possible cause of the presence of an extra band in the results of DNA extraction and PCR…

A: This finding suggests that formation of multiple bands in non-denaturing gel electrophoresis is a…

Q: in the cloning vector, what would be the plausible impacts if a mutation at the Ori site renders it…

A: The origin of replication (ori) and all of its regulatory components make up the replicon. DNA…

Q: What is a positive and negative control and what are their roles in PCR?

A: In polymerase Chain Reaction or PCR a given sample of DNA that contains very very small quantity of…

Q: What detection method could be used to determine the mutagen added to a microbial culture resulting…

A: The mutagens are chemical mixtures or a type of radiation that cause irreversible and heritable…

Q: Describe how Ames Test works to identify carcinogenic using back mutations (reversible)

A: The Ames test is a widely employed method that uses bacteria to test whether a given chemical can…

Q: What is knockdown gene analysis? How is this different from knockout analysis?

A: Gene knockdown is a method in which temporarily stop or decrease the expression of one or more…

Q: What Genetic Conditions Are Candidates for Treatment by GeneTherapy?

A: The Genetic Conditions Are Candidates for Treatment by Gene Therapy are: • These cells can be…

Q: What is meant by the term genetic testing? How do testing at theprotein level and testing at the DNA…

A: Medical genetics is one of the branches of medicine, which involves the management and diagnosis of…

Q: Why are temperature-sensitive mutations useful for uncovering thefunction of a gene?

A: Mutations are alterations in the DNA sequence due to the mistakes during cell division, exposure to…

Q: why are DTT and Mg added to PCRs? what is their affect and role in there?

A: The process of obtaining multiple copies of a known DNA sequence is known as gene amplification. It…

Q: If you wanted to make a mouse model for any of the following human genetic conditions (a–d),…

A: Since we only answer up to 3 sub-parts, we’ll answer the first three. Please resubmit the question…

Q: How to identify all the somatic mutationswithin tumors ?

A: A somatic mutation is a modification within the desoxyribonucleic acid sequence of a somatic cell of…

Q: What does MEGA stand for and what is this assay used to determine? How to do perform the assay?

A: Many assisted reproductive techniques are widely used around the globe.

Q: How can telomerase-related aging be addressed therapeutically?

A: Introduction DNA is packed inside the cell in the form of chromosomes and chromatin. The…

Q: How can site-directed mutagenesis be useful to enzymologists?

A: Mutagenesis is the biological process of changing genetic information or DNA of an organism and that…

Q: Site directed mutagenesis is used to: 1.determine the critical base per sequences in a Genome 2.…

A: DNA consists of double strands of sequentially arranged nucleotides. The nucleotide sequence present…

Q: What are knockout mice, how are they produced, and for what are they used?

A: The gene knockout is a mechanism in which one of the genes of an organism is made inoperative. The…

Q: What is meant by a transgenic animal?

A: Recombinant DNA technology involves integrating DNA molecules from two different species and…