What is similar between molecular cloning and PCR? Q2) What is different from molecular cloning and PCR? Q3) What ‘horizontal gene transfer’ technique is utilized most often in cloning? (conjugation, transformation or transduction)
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Q1) What is similar between molecular cloning and PCR?
Q2) What is different from molecular cloning and PCR?
Q3) What ‘horizontal gene transfer’ technique is utilized most often in cloning? (conjugation, transformation or transduction)
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Solved in 2 steps with 1 images
- Do all of them True/False 31) The process by which an electrical charge is used to introduce DNA into a cell to produce a transgenic organism is called electroporation.Answer: 32) Reproductive cloning is used to produce large amounts of mammalian proteins from transgenic agricultural animals such as cattle.Answer: 33) In gene addition, homologous recombination is used to remove the original gene and replace it with the cloned gene.Answer: 34) All stem cells have the potential to differentiateAnswer: 35) A bone marrow transplant involves the transfer of multipotent stem cellsAnswer: 36) The fact that in mammalian systems multiple genes may compensate for the loss of a gene is called gene redundancy.Answer:Q7) What are things that are common in cloning vectors? Why are they useful?Q8) Using the pCR 2.1 vector, what antibiotics would a cell with the vector be resistant to?Q9) What does B-galactosidase do?Following are four processes common to most cloning experiments: a) transforming bacteria b) plating bacteria on selective medium c) cutting DNA with restriction endonucleases d) ligating DNA fragments Place components of this list in the order in which they would most likely occur during a cloning experiment.
- 1) Follow the steps involved in DNA or gene cloning 2) In what process do bacteria take up the recombinant plasmid DNA 3) What are the advantages or replications of gene cloning?In PCR , the primers will determine which gene will amplified (copied) . in lab we’re doing qRT- PCR using PAL primers and pair of primers for an RRNA gene what would happen is we setup a PCA and used primers for myostatin - what gene would be amplified (copied) ? A) any gene could be Amplified B) myostatin C) PAL D) no gene would be amplified-What is DNA cloning? What is the common organism that the scientists use to do cloning (explain briefly how it is done) -What is Gene of Interest? -What are the ethical issues on Biotechnology?
- 5) Below is an image that shows both reproductive and therapeutic cloning. Use this image to answer compare and contrast therapeutic and reproductive cloning. Are they used for similar means…etc. Once you have done that answer the question below. a) There are two types of therapeutic cloning. What are they and how are they different?Which method is used to obtain mutants that grow under conditions that the wild type parent cannot grow? a)indirect selction b) direct selection c) screening for possible mutagen ( carcinogens) d) replica platingChoose the phrase that best describes the role of a cloning vector. A) Separates fragments of DNA B) Detects the presence of specific DNA sample C) Isolates DNA from a donor organism D) Screens for recombinants that have been successfully transformed E) Introduces DNA into a cloning host
- What is the difference between molecular cloning and reproductive cloning?Cloning vectors are not just limited to bacterial plasmids. Bacteriophages and M13 phage vectors are also commonly utilized in the cloning process. State any five (5) key criteria to be an effective cloning vector.With the use of well-illustrated diagrams, reconstruct the entire cloning process by explaining different stages of the cloning process that involves the following:a. Isolation of target DNA fragments (often referred to as inserts)b. Ligation of inserts into the plasmid, creating recombinant molecules c. Transformation of recombinant plasmids into bacteria or other suitable host for propagationd. Screening/selection of hosts containing the intended recombinant plasmid. For this stage(d), discuss the importance of a second marker that can be used for screening of genomic DNA for colonies containing the pka-1 under the principle of insertional inactivation. This should be properly explained using all the attributes of the plasmid described above.